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Primed and naïve marmoset pluripotent stem cells as a model for primate development

ABSTRACT: Primed and naïve marmoset pluripotent stem cells as a model for primate development

PROVIDER: PRJEB39395 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4342080_1.fastq.gz	Fastqsanger.gz
	ERR4342080_2.fastq.gz	Fastqsanger.gz
	ERR4342090_1.fastq.gz	Fastqsanger.gz
	ERR4342090_2.fastq.gz	Fastqsanger.gz
	ERR4342100_1.fastq.gz	Fastqsanger.gz

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Primed and naïve marmoset pluripotent stem cells as a model for primate development

Project description:We derived primed pluripotent stem cell cultures (PSCs) from marmoset blastocyst using conventional human PSC culture conditions (KSR/bFGF on feeders). Naïve marmoset PSCs were established by chemical resetting. In addition, we generated forebrain-derived cells from the frontal lobe of a marmoset neonate. Individual cells were manually isolated with a mouth pipette and subjected to single-cell full-lengths transcriptome profiling using a modified version of Smart-Seq2.

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Project description:The presence of aneuploidy (chromosomal abnormalities) in embryos is one of the main barriers to successful human reproduction, causing nearly 50% of early miscarriages. Despite its high incidence in human embryos, our understanding of the molecular mechanisms and cellular developmental fate of aneuploid cells is limited. In this study, we demonstrate that the New World primate marmoset (Callithrix jacchus) is an excellent model for investigating aneuploidy. We developed an efficient method to generate naïve marmoset embryonic stem cells (cj-ESCs) from cj-ESCs in the primed state, resulting in cells that resemble preimplantation epiblast-like cells and possess ground-state pluripotency, allowing them to contribute to all cell lineages, including trophoblasts, and form blastocyst-like structures. We observed that naïve marmoset ESCs are highly chromosomally unstable, making them prone to aneuploidy. We identified inherently less stringent mitotic checkpoints in ground-state embryonic stem cells as a potential cause of chromosomal instability, shedding light on the mechanisms underlying the prevalence of aneuploidy in primates during early embryonic development. Our findings establish that naïve marmoset ESCs faithfully replicate the cellular phenotypes of early embryonic cells, offering a powerful tool for studying the fate of aneuploid cells post-implantation in vivo.

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Genome-wide transcriptome analysis of integrin β1 inhibited primed and naïve human induced pluripotent stem cells.

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