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Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset

ABSTRACT: Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset

PROVIDER: PRJEB39815 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4451030_1.fastq.gz	Fastqsanger.gz
	ERR4451030_2.fastq.gz	Fastqsanger.gz
	ERR4451031_1.fastq.gz	Fastqsanger.gz
	ERR4451031_2.fastq.gz	Fastqsanger.gz
	ERR4451032_1.fastq.gz	Fastqsanger.gz

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Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset

Project description:To better understand the epigenetic mechanism underlying pubertal onset, the hypothalamic genome-wide DNA methylation and hydroxymethylation patterns as well as the transcription profiles in mouse arcuate nucleus at early and late pubertal stages were explored. Female mice have been widely used in multiple studies on pubertal development as they present the similar molecular behaviors in HPG axis and stable cycles of menstrual calendar like human. Hypothalamic ARC underwent a huge epigenetic and genetic reprogramming to adapt to the response and feedback on sexual hormones during the stages of early pubertal (2-5-week of age) and late puberty (5-8-week of age) . We harvested 4- and 8-week hypothalamic ARC and employed RNA-seq, reduced representation bisulfite sequencing (RRBS) and hydroxymethylation profiling (RRHP) on a genome-wide scale. We identified a large number of differential expressed genes (DEGs) and differential 5(h)mC signals across the whole genome. We discovered novel connections between DNA (hydroxyl)methylated modification and gene expression, emphasizing the importance of epigenetic alterations in regulating transcription in puberty onset.

2020-08-21 | E-MTAB-9421 | biostudies-arrayexpress

Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset.

2020-08-20 | E-MTAB-9420 | biostudies-arrayexpress

Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset.

Project description:Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset.

| PRJEB39790 | ENA

Chromatin accessibility in mouse hypothalamic arcuate nucleus in puberty onset

Project description:To better understand the epigenetic mechanism underlying pubertal onset, the hypothalamic genome-wide chromatin accessibility patterns in mouse arcuate nucleus at early and late pubertal stages were explored. Female mice have been widely used in multiple studies on pubertal development as they present the similar molecular behaviors in HPG axis and stable cycles of menstrual calendar like human. Hypothalamic ARC underwent a huge epigenetic and genetic reprogramming to adapt to the response and feedback on sexual hormones during the stages of early pubertal (2-5-week of age) and late puberty (5-8-week of age) . We harvested 4- and 8-week hypothalamic ARC and employed ATAC-seq on a genome-wide scale. Combined with previous RRBS, RRHP and RNA-seq, the connections between DNA (hydroxyl)methylation in retroelements and gene expression were studied, emphasizing the importance of epigenetic alterations in regulating transcription in puberty onset.

2021-04-02 | E-MTAB-10263 | biostudies-arrayexpress

Chromatin accessibility in mouse hypothalamic arcuate nucleus in puberty onset

Project description:Chromatin accessibility in mouse hypothalamic arcuate nucleus in puberty onset

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Proteomic profiling of the hypothalamic arcuate nucleus of bull calves offered either a high or moderate plane of nutrition in early life

Project description:Enhanced nutrition during the early calfhood period has been shown to hasten the onset of puberty in bull calves. The hypothalamic-pituitary-ovarian biochemical signalling axis regulates reproductive development in bulls, with the dynamics of the arcuate nucleus region of the hypothalamus, in particular, central to mediating the relationship between metabolic status with reproductive development. However, the precise molecular mechanisms regulating the influence of metabolic status on this signalling axis in bull calves is yet to be fully elucidated. The objective of this study was to determine the effect of an enhanced plane of nutrition during early life, up to 12 weeks of age, on the proteomic profile of the arcuate nucleus of bull calves. Bull calves were offered either a high or moderate plane of nutrition.

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Integrative proteomic and phosphoproteomic analysis in the female goat hypothalamus to study the onset of puberty

Project description:Puberty marks the end of childhood and achieve sexual maturation and fertility. The role of hypothalamic proteins in regulating puberty onset is unclear. We performed a comprehensive differential proteomics and phosphoproteomics analysis in prepubertal and pubertal goats to determine the roles of hypothalamic proteins and phosphoproteins during the onset of puberty.

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Effect of enhanced early life nutrition on hypothalamic arcuate nucleus miRNA transcriptome in bull calves.

Project description:The aim of this study was to measure the impact of contrasting feeding regimes in the first 12 wk of life, on the molecular control of hypothalamic arcuate nucleus tissue in bull calves. Holstein bull calves were designated to high (HI; n=15) or moderate (MOD; n=12) dietary groups, with diets designed to provoke growth rates of 1.0 and 0.5 kg/day, respectively. At 12 wk of age, all calves were euthanized, and hypothalamic arcuate nucleus tissue harvested. RNA was extracted from the hypothalamic arcuate nucleus and used for miRNAseq.

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