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MicroRNA profiling of female and male mouse pituitary gland across pubertal transition

ABSTRACT: microRNA profiling of female and male mouse pituitary gland across pubertal transition

PROVIDER: PRJEB39965 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4471840.fastq.gz	Fastqsanger.gz
	ERR4471850.fastq.gz	Fastqsanger.gz
	ERR4471860.fastq.gz	Fastqsanger.gz
	ERR4471870.fastq.gz	Fastqsanger.gz
	ERR4471880.fastq.gz	Fastqsanger.gz

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microRNA profiling of female and male mouse pituitary gland across pubertal transition

Project description:The pituitary gland exhibits sex differences in its function. Diseases associated with dysregulation of the pituitary are also sex-biased in prevalence. Previous qPCR profiling of puberty-related genes in the pituitary gland revealed increasingly sex-biased expression of genes profiled across pubertal transition. Here, we performed small RNA-seq on total RNA extracted from C57BL/6J mouse pituitary gland of both sexes mice at 4 ages spanning pubertal transition (postnatal days 12, 22, 27, 32) (6 replicates per sex at each age) to examine microRNA (miRNA) regulation of sex differences in gene expression observed. Total RNA was sent to SickKids TCAG core to construct small RNA-seq libraries using NEBNext Small RNA Library Prep Kit according to manufacterer’s protocol. Resulting single-end libraries were sequenced at TCAG core on the Illumina HiSeq 2500 v4 flow cell with SR50 bp. Data obtained was processed using miRDeep2 pipeline to align small RNA-seq reads to the mouse genome (mm10) and to filter for miRNAs.

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3'-UTR-seq of female and male mouse pituitary gland across pubertal transition

Project description:3'-UTR-seq of female and male mouse pituitary gland across pubertal transition

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Proteomic analysis of human anterior pituitary gland

Project description:The regulation of pituitary function via the hypothalamus and via intra-pituitary connections represents a complex system. Though hormones secreted from the pituitary glands have been well studied, overall information of proteins expressed in the pituitary glands is very limited. Protein expression profiling of normal pituitary tissue may lead to discovery of novel proteins playing an important role in the physiology of pituitary glands and can lead to better understanding of pituitary gland diseases. We aimed to carry out systematic proteomic profiling of adenohypophysis from human pituitary glands using high-resolution Fourier transform mass spectrometer. A total of 2,175 proteins were identified in this study of which, 105 proteins were identified for the first time as compared to high throughput proteomic-based studies from human pituitary glands. The comprehensive list of proteins identified in this study will facilitate the better understanding the role of this important gland in health and disease.

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