Project description:The influence of temperatures on dormant bud gene expression was analyzed during an eighteen-day period in January 2016 (January 14th to 31st) when daily temperatures ranged between 13 ºC and -15 ºC. This study collection period captured three different natural consecutive temperature events that fluctuated above and below 0 ºC or remained constantly below 0 ºC. Dormant buds were collected from two different wine grapevine cultivars, Cabernet Franc’ and ‘Riesling’. The buds were collected from Ravines Wine Cellar vineyards in Geneva, New York (42.843898, -77.003488. There were two 84-hour diurnal cycles (DC) that included temperatures fluctuating above and below 0 ºC (DC1: Jan 14 to Jan 17; DC2: Jan 24 to Jan 27) (Figure 1, Table 1). In DC1 and DC2, samples were collected 3 times/day, during the dark periods (6:00 and 18:00) and once in the light at warmest part of the day (14:00). The DC1 and DC2 periods bracketed four days (96 h) of constant daily temperatures below 0 ºC (Jan 18 to Jan 22). This constant below 0 ºC period was sampled in the light period only (14:00) (Figure 2, Table 1). Three replicate bud samples, each consisting of 3 buds per replicate were collected, each replicate from a separate vine for Reisling and Cabernet franc. Bud samples were immediately plunged into liquid nitrogen and stored at -80 ºC until RNA extraction.
Project description:By characterizing the cell wall proteomes of different sugarcane organs (leaves and stems) at two developmental stages (young vs mature/apical vs basal), it was possible to address unique characteristics in each of them. Four-month-old leaves showed a higher proportion of oxido-reductases and proteins related to lipid metabolism (LM), besides a lower proportion of proteins acting on polysaccharides, in comparison to four-month-old internodes. It was possible to note that sugarcane leaves and young stems have the highest LM rate than all species, which was assumed to be linked to cuticle formation. The data generated enriched the number of cell wall proteins (CWPs) identified in sugarcane, reaching 277. To our knowledge, sugarcane has now the second higher coverage of monocot CWP in plants