Project description:The influence of temperatures on dormant bud gene expression was analyzed during an eighteen-day period in January 2016 (January 14th to 31st) when daily temperatures ranged between 13 ºC and -15 ºC. This study collection period captured three different natural consecutive temperature events that fluctuated above and below 0 ºC or remained constantly below 0 ºC. Dormant buds were collected from two different wine grapevine cultivars, Cabernet Franc’ and ‘Riesling’. The buds were collected from Ravines Wine Cellar vineyards in Geneva, New York (42.843898, -77.003488. There were two 84-hour diurnal cycles (DC) that included temperatures fluctuating above and below 0 ºC (DC1: Jan 14 to Jan 17; DC2: Jan 24 to Jan 27) (Figure 1, Table 1). In DC1 and DC2, samples were collected 3 times/day, during the dark periods (6:00 and 18:00) and once in the light at warmest part of the day (14:00). The DC1 and DC2 periods bracketed four days (96 h) of constant daily temperatures below 0 ºC (Jan 18 to Jan 22). This constant below 0 ºC period was sampled in the light period only (14:00) (Figure 2, Table 1). Three replicate bud samples, each consisting of 3 buds per replicate were collected, each replicate from a separate vine for Reisling and Cabernet franc. Bud samples were immediately plunged into liquid nitrogen and stored at -80 ºC until RNA extraction.
Project description:LncRNAs and mRNAs expression profile of MEFs comparing senescent cells with young cells. The microarray with coverage of 41597 mouse lncRNAs and 35291 mouse mRNAs. In the study presented here, six samples were analyzed. Biological replicates: three Senescent MEFs, three Young MEFs, independently grown and harvested.
Project description:We utilized whole genome sequencing of mRNA (RNA-seq) to understand the extent to which the senescence-associated secretory phenotype is regulated by p38MAPK Examination of replicates of young, senescent or p38MAPK-inhibited senescent BJ human foreskin fibroblasts.
Project description:Malignant melanoma might develop from melanocytic nevi in which the growth-arrested state has been broken. We analyzed the gene expression of young and senescent human melanocytes in culture. In this dataset we include the expression data of young and senescent human melanocytes.
Project description:This study compares global gene expression profiles of human umbilical vein endothelial cells (HUVECs) under three conditions: young, replicative senescent, and premature senescent states. RNA sequencing was performed to identify transcriptional changes associated with endothelial aging. The dataset provides gene-level counts and processed expression matrices for downstream analysis.
Project description:Cytokinins are plant hormones with biological functions ranging from coordination of plant growth and development to the regulation of senescence. A series of 2-chloro-N6-(halogenobenzylamino)purine ribosides was prepared and tested for cytokinin activity in selected bioassays. Several compounds showed significant activity, especially in delaying senescence in detached wheat leaves. We used microarrays to gather information about the reprogramming of gene transcription when senescent Arabidopsis leaves were treated with selected C2-substituted aromatic cytokinin ribosides that showed high activity in the senescence bioassay. Arabidopsis senescent leaves were treated with cytokinins and subsequently used for RNA extraction and hybridization on Affymetrix microarrays. 21-days old Arabidopsis leaves were treated with the appropriate cytokinin or left untreated (DMSO only).