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RNAseq of human SAOS-2 cell lines expressing different p53 cooperativity mutants ectopically over a p53 null background

ABSTRACT: RNAseq of human SAOS-2 cell lines expressing different p53 cooperativity mutants ectopically over a p53 null background

PROVIDER: PRJEB43617 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR5457660.fastq.gz	Fastqsanger.gz
	ERR5457670.fastq.gz	Fastqsanger.gz
	ERR5457680.fastq.gz	Fastqsanger.gz
	ERR5457661.fastq.gz	Fastqsanger.gz
	ERR5457671.fastq.gz	Fastqsanger.gz

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Characterization of the p53 cistrome - DNA binding cooperativity dissects p53's tumor suppressor functions

Project description:p53 wildtype or complementing DNA binding cooperativity versions (EE, RR, EE/RR) were overexpressed in SAOS cells and 18h later harvested to perform ChIP with an p53-specific antibody. The enriched DNA fragments were purified and identified by high throughput sequencing.

2014-02-14 | E-MTAB-1394 | biostudies-arrayexpress

Characterization of the p53 cistrome - DNA binding cooperativity dissects p53's tumor suppressor functions - Expression profiling

Project description:P53 wildtype or complementing DNA binding cooperativity versions (p53EE, p53RR, p53EE/RR) were overexpressed in SAOS cells. After 18h cells were harvested and subjected to expression profiling.

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Effects of PRIMA-1 treatment on Saos-2 p53-null and Saos-2 p53-His273 cells

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2009-04-15 | E-GEOD-15367 | biostudies-arrayexpress

Expression analysis of Saos-2 cells expressing p53-His273

Project description:Our compound (PRIMA) selectively kills tumor cells expressing mutant p53. Here in our study we decided to investigate the impact of our compound on the transcriptome of p53 mutant-expressing cells, Saos2-His273. We plated Saos-2-His273 cells and treated them with our compound for 6h or 12h. We then harvested the cells, extracted the RNA, and followed the protocol to run affymetrix array to analyse the impact of our drug on the whole transcriptome of cells expressing mutant p53.

2009-04-15 | E-GEOD-15368 | biostudies-arrayexpress

Saos-2 vs. Saos-2-2 cells

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