Project description:To investigate the effect of TMIGD2 on transcriptional functions in human AML cell line, we performed gene expression profiling analysis using data obtained from RNA-seq of HEL cells upon TMIGD2 knockdown.
Project description:To understand the role of LSD1 in regulating histone H3K4 methylation status, ChIP-seq analyse of mono- and di-methylated H3K4 in LSD1-KD HEL cells were performed. The analyses revealed demethylation of H3K4me1 and H3K4me2 by LSD1 at regulatory regions including CEBPA gene enhancer.
Project description:compare the gene expression profile between cep701 treated HEL cells with shPRMT5 knockingdown HEL cells. HEL cells contain homologous alells with mutation Jak2V617F. We found JAK2V617F can inactivate PRMT5 activity by directly phosphorylating PRMT5 through histone methylation.
Project description:Fibroblast Growth Factor 12 (FGF12), a non-secretory member of the FGF homologous factor family, plays a pivotal role in modulating intracellular signaling and cellular homeostasis. In this study, we successfully established stable FGF12-overexpression cell lines using HEL cell line via lentiviral transduction. To rigorously evaluate the phenotypic and molecular shifts, an empty vector Negative Control (NC) group was simultaneously generated. By integrating high-throughput transcriptomic analysis and biochemical assays, we aimed to explore the molecular landscape altered by FGF12 upregulation. Our preliminary findings indicate significant perturbations in p53 signaling ,providing new insights into the regulatory network of FGF12 and its potential as a therapeutic target in AML.
2026-03-01 | GSE318240 | GEO
Project description:Whole human genome methylation analysis of the HEL cell line using nanopore sequencing
| PRJEB45078 | ENA
Project description:Whole human genome methylation analysis of the HEL cell line using nanopore sequencing
Project description:This study aims to determine the chromosome content and organisation of two myeloid leukaemia cell lines, HEL and U937. This will be done not only with SNP array data to determine breakpoints and copy number of copy number aberrations, but also with FISH (multicolour FISH, multicolour banding, centromere and single locus FISH) to identify translocation partners, chromosome organistion, centromere content, and provide some information on genome evolution in the cell line. Although several HEL SNP array karyotypes have been published and are available online the information here shows that there are some differences, and the additional FISH tests provide a greater depth of information on genome organisation and derivation of the abnormal chromosomes. The U937 cell line was also studied using DNA from fresh and fixed specimens for comparison of the quality of the SNP array data. Data from cells fixed using standard cytogenetic fixative (3:1 methanol:acetic acid) were compared to data from cells processed directly from tissue culture.
Project description:We used the nanopore Cas9 targeted sequencing (nCATS) strategy to specifically sequence 125 L1HS-containing loci in parallel and measure their DNA methylation levels using nanopore long-read sequencing. Each targeted locus is sequenced at high coverage (~45X) with unambiguously mapped reads spanning the entire L1 element, as well as its flanking sequences over several kilobases. The genome-wide profile of L1 methylation was also assessed by bs-ATLAS-seq in the same cell lines (E-MTAB-10895).
