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ChIP-seq of wild-type SOX18 and a dominant-negative variant (SOX18RaOp) in HeLa cells

ABSTRACT: ChIP-seq of wild-type SOX18 and a dominant-negative variant (SOX18RaOp) in HeLa cells

PROVIDER: PRJEB45796 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR6090133.fastq.gz	Fastqsanger.gz
	ERR6090134.fastq.gz	Fastqsanger.gz
	ERR6090135.fastq.gz	Fastqsanger.gz
	ERR6090136.fastq.gz	Fastqsanger.gz
	ERR6090137.fastq.gz	Fastqsanger.gz

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ChIP-seq of wild-type SOX18 and a dominant-negative variant (SOX18RaOp) in HeLa cells

Project description:We investigated differences in the chromatin-binding profile of SOX18 (mouse) and its dominant-negative isoform SOX18RaOp, and how SOX18RaOp alters the binding location of SOX18. HeLa cells were transfected to generate 3 different conditions: 1. myc-SOX18, 2. myc-SOX18RaOp and 3. myc-SOX18 with untagged-SOX18RaOp. Each condition was performed in duplicate (6 samples total). An anti-Myc antibody was used to perform the pull-down. Background (input) was assessed by pooling cells transfected with each condition and sequencing without immunoprecipitation.

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