Genomic analysis reveals new Integrative Conjugal Elements and transposons in GBS conferring antimicrobial resistance
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PROVIDER: PRJEB59039 | ENA |
REPOSITORIES: ENA
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Project description:Streptococcus agalactiae or group B streptococcus (GBS) is a leading cause of neonatal sepsis and increasingly found as an invasive pathogen in older patient populations. Beta-lactam antibiotics remain the most effective therapeutic with resistance rarely reported, while the majority of GBS isolates carry the tetracycline resistance gene tet(M) in fixed genomic positions amongst five predominant clonal clades. In the UK, GBS resistance to clindamycin and erythromycin has increased from 3% in 1991 to 11.9% (clindamycin) and 20.2% (erythromycin), as reported in this study. Here, a systematic investigation of antimicrobial resistance genomic content sought to fully characterise the associated mobile genetic elements within phenotypically resistant GBS isolates from 193 invasive and non-invasive infections of UK adult patients collected during 2014 and 2015. Resistance to erythromycin and clindamycin was mediated by erm(A) (16/193, 8.2%), erm(B) (16/193, 8.2%), mef(A)/msr(D) (10/193, 5.1%), lsa(C) (3/193, 1.5%), lnu(C) (1/193, 0.5%), and erm(T) (1/193, 0.5%) genes. The integrative conjugative elements (ICEs) carrying these genes were occasionally found in combination with high gentamicin resistance mediating genes aac(6')-aph(2″), aminoglycoside resistance genes (ant(6-Ia), aph(3'-III), and/or aad(E)), alternative tetracycline resistance genes (tet(O) and tet(S)), and/or chloramphenicol resistance gene cat(Q), mediating resistance to multiple classes of antibiotics. This study provides evidence of the retention of previously reported ICESag37 (n = 4), ICESag236 (n = 2), and ICESpy009 (n = 3), as well as the definition of sixteen novel ICEs and three novel transposons within the GBS lineage, with no evidence of horizontal transfer.
| S-EPMC10044541 | biostudies-literature
Project description:<p>Antimicrobial resistance (AMR) is one of the most urgent challenges in public health, and the horizontal transfer of antibiotic resistance genes (ARGs) mediated by mobile genetic elements (MGEs) drives its widespread global dissemination. Although integrative conjugative elements (ICEs) outnumber conjugative plasmids, research on ICE-mediated horizontal gene transfer (HGT) remains largely lacking, especially concerning SXT ICEs, which are highly prevalent, pose significant risks, and are directly related to human health. Cyromazine is a widely used feed additive, with high detection rates in the feces of farmed animals and related environments. It has also been shown to increase the abundance of ARGs, and the potential driving mechanisms urgently require further exploration. Thus, we investigated the impact of cyromazine on SXT ICE-mediated ARGs transfer and elucidated its underlying mechanisms. Our study demonstrates that cyromazine promotes SXT ICE conjugative transfer both intra- and inter-species. This facilitating effect confirmed in mouse intraperitoneal and intestinal conjugation models. The potential mechanisms include cyromazine-induced ROS accumulation and triggering of the SOS response, which could promote SXT ICE excision and activate conjugation-related operons. Furthermore, enhanced energy metabolism and altered lipid metabolism further facilitate SXT ICE transfer. Collectively, our study partially fills the gap in understanding ICE-mediated AMR transmission dynamics, and highlighting that the exacerbated AMR crisis is associated with cyromazine-stimulated high-frequency SXT ICE conjugation in vitro and in vivo.</p>
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Project description:The aim of this study was to investigate the response of human brain endothelial cells to bacterial (group B streptococcus, GBS) infection. Results: GBS WT strain infection results in a specific gene induction pattern that is different from the pilA mutant, but not other mutants such as pilB and srr-1. Conclusion: These findings suggest that the GBS PilA protein contributes to gene induction in brain endothelium.
2011-09-06 | E-MTAB-765 | biostudies-arrayexpress
Project description:Global regulatory roles of the TCS response regulator CovR in GBS serotype 1a A909 Keywords: Strain comparison; global regulation
2007-10-18 | GSE9370 | GEO
Project description:We report the characterization of the major regulator of virulence gene expression (CovR) in Group B Streptococcus. The ChIP-seq experiments define the binding of CovR on the chromosome of the BM110 strain, a representative of the hypervirulent GBS lineage responsible of neonatal meningitis. Regulatory evolution of CovR signaling was investigated by comparing ChIP-seq done in parallel in a second GBS clinical isolate (NEM316) not belonging to the hypervirulent lineage.
2021-08-23 | GSE158046 | GEO
Project description:Armatimonadetes bacterium GBS isolate:GBS Genome sequencing and assembly
| PRJNA285317 | ENA
Project description:Streptococcus agalactiae (Group B Streptococcus, GBS) can colonize the human vaginal tract leading to both superficial and serious infections in adults and neonates. To study bacterial colonization of the reproductive tract in a mammalian system, we employed a murine vaginal carriage model. Using RNASeq, the transcriptome of GBS growing in vivo during vaginal carriage was determined. Over one-quarter of the genes in GBS were found to be differentially regulated during in vivo colonization as compared to laboratory cultures. A two-component system (TCS) homologous to the staphylococcal virulence regulator SaeRS was identified as being up-regulated in vivo. One of the SaeRS targets, pbsP, a proposed GBS vaccine candidate, was shown to be important for colonization of the vaginal tract. A component of vaginal lavage fluid acted as a signal to turn on pbsP expression via SaeRS. These data demonstrate the ability to quantify RNA expression directly from the murine vaginal tract and identify novel genes involved in vaginal colonization by GBS. They also provide more information about the regulation of an important virulence and colonization factor of GBS, pbsP, by the TCS SaeRS.
2018-02-06 | GSE109680 | GEO
Project description:Global regulatory roles of the TCS response regulator CovR in GBS serotype 1a A909 Keywords: Strain comparison; global regulation
2010-08-14 | GSE12463 | GEO