Project description:Plasmids are widely used across molecular biology and are becoming increasingly valuable products, but robust plasmid replication is held back by stability issues in the host. This study investigated how Escherichia coli responds to plasmid stress at the transcriptional level by modulating plasmid copy number, plasmid size, selection marker and carbon source. This GEO contains controls from this study alone.
Project description:The IncX3 plasmid is a primary vector for disseminating carbapenem resistance globally, yet the chromosomal regulatory mechanisms governing its fitness cost remain poorly understood. Our previous work identified the stringent starvation protein SspA as a modulator of IncX3 plasmid fitness in Escherichia coli via flagellar biosynthesis. Here, we elucidate the global regulatory role of SspA and verify whether its effect is mediated through the master regulator H-NS
Project description:To demonstrate plasmid transferability by conjugation, cultures of the donor S. Infantis, and recipient Escherichia coli (E. coli) K12 were mated. S. Infantis and transconjugant were screened for resistance genes.
Project description:Transcription profiles in BL21, BL21/pOri1 and BL21/pOri2 were analysed using DNA microarray technology. BL21, BL21/pOri1 or BL21/pOri2 strains were cultured at chemostat status and harvested after the cultivation arrived steady status. Keywords: Effects of plasmid DNA on Escherichia coli metabolism
