IMP2-TRIBE
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB71750 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:IGF2BP2 (IMP2) is an RNA binding protein contributing to tumorigenesis across various cancers as well as to metabolic disorders. With the growing interest in drug discovery efforts targeting IMP2, an understanding of its structural properties and biological behavior are needed. Thus far, only partial structural information was available based on selected domains purification. In this study we used a combination of mass photometry, Hydrogen-Deuterium exchange-mass spectrometry, small angle X-ray scattering, and additional biophysical experiments to investigate the behavior of full-length IMP2 protein in presence or absence or RNA. We found that full-length IMP2 forms multiple oligomeric states and predominantly adopts a dimer conformation. Interestingly, the dimer is formed in a head-to-tail orientation by KH34 and RRM1 domains. In the presence of a known IMP2-binding RNA target, we observed a pseudo-symmetric dimer much different from its RNA unbound state, with the KH12 domains of each IMP2 molecule forming the dimeric interface. Additionally, we observed that IMP2 oligomer species, including dimers and higher-order oligomers, are sensitive to environmental conditions such as ionic strength as well as the presence or absence of RNA. Our results provide the first insight into IMP2 homodimerization, which will provide novel opportunities for disrupting its function towards more effective IMP2 inhibitors.
2026-05-25 | PXD049065 | Pride
Project description:The goal of this study is to understand how IMP2 contributes to T2D traits by regulating pancreatic beta cell growth and function. Islets from control and Rip2;IMP2 mice were generated by deep sequencing. In additon, we also perfomred IMP2 RIP-Seq to identify IMP2 targets.
2023-06-11 | GSE152424 | GEO
Project description:HITS-CLIP is performed for the RNA-binding protein, IMP2/IGF2BP2, from E13.5 mouse brain.
2016-06-06 | E-MTAB-3636 | biostudies-arrayexpress
Project description:RNA-based regulatory mechanisms play important roles in the development and plasticity of neural circuits and neurologic disease. Developing axons provide a well suited model to study RNA-based regulation, and contain specific subsets of mRNAs that are locally translated and have roles in axon pathfinding. However, the RNA-binding proteins involved in axon pathfinding, and their corresponding mRNA targets, are still largely unknown. Here we find that the RNA-binding protein IMP2 (Igf2bp2) is strikingly enriched in developing axon tracts, including in spinal commissural axons. We used the HITS-CLIP approach to perform a genome-wide identification of RNAs that interact directly with IMP2 in the native context of developing brain. This IMP2 interactome was highly enriched for mRNA targets related to axon guidance. Accordingly, IMP2 knockdown in the developing spinal cord led to strong defects in commissural axon trajectories at the midline intermediate target. These results reveal a highly distinctive axonal enrichment of IMP2, show that it interacts with a network of axon guidance-related mRNAs, and reveal its requirement for normal axon pathfinding during vertebrate development. CLIP-seq
2016-08-02 | E-GEOD-83822 | biostudies-arrayexpress
Project description:Chaperone-mediated autophagy (CMA) selectively targets proteins for lysosomal degradation. During aging, levels of LAMP2A (lysosome-associated membrane protein 2A), a limiting CMA component, decline. Here we show that insulin-like growth factor 2 mRNA-binding protein 2 (IMP2), is a post-transcriptional regulator of CMA that binds Lamp2a mRNA and inhibits its translation, leading to decreased CMA activity and that IMP2 depletion or pharmacological inhibition effectively upregulate CMA. We identified a fraction of IMP2 at the lysosomal surface and using single-molecule fluorescence in situ hybridization, demonstrated its contribution to the lysosomal degradation of Lamp2a mRNA. IMP2 levels and distribution change with age and in response to dietary lipid challenges, both conditions known to reduce CMA activity. We propose that the increase in IMP2 levels under these conditions enhances Lamp2a RNA degradation, thereby contributing to the decline of CMA. Our data highlight IMP2 as a physiological regulator of CMA activity and potential target for gerotherapeutic interventions.
2026-07-15 | GSE260682 | GEO
Project description:RNA-based regulatory mechanisms play important roles in the development and plasticity of neural circuits and neurologic disease. Developing axons provide a well suited model to study RNA-based regulation, and contain specific subsets of mRNAs that are locally translated and have roles in axon pathfinding. However, the RNA-binding proteins involved in axon pathfinding, and their corresponding mRNA targets, are still largely unknown. Here we find that the RNA-binding protein IMP2 (Igf2bp2) is strikingly enriched in developing axon tracts, including in spinal commissural axons. We used the HITS-CLIP approach to perform a genome-wide identification of RNAs that interact directly with IMP2 in the native context of developing brain. This IMP2 interactome was highly enriched for mRNA targets related to axon guidance. Accordingly, IMP2 knockdown in the developing spinal cord led to strong defects in commissural axon trajectories at the midline intermediate target. These results reveal a highly distinctive axonal enrichment of IMP2, show that it interacts with a network of axon guidance-related mRNAs, and reveal its requirement for normal axon pathfinding during vertebrate development.
2016-08-02 | GSE83822 | GEO
Project description:Using IMP2-deficient mice to study the role of the RNA-binding protein (RBP) IMP2 in models of autoimmunity, namely autoimmune glomerulonephritis
2021-07-02 | GSE178710 | GEO
Project description:Data showing the late 2-cell-stage, control embryos (Imp2♀+/♂+) and Imp2-knockout embryos (Imp2♀−/♂+) for HPLC MS/MS analysis. 3 replicates were performed using 330 embryos per group.
2019-11-12 | PXD010397 | Pride
Project description:Cancer stem cells (CSC) dictate tumor cell heterogeneity in diverse cancer types and arise, in part, from microRNA (miRNA)-dependent alteration of gene expression. The let-7 miRNA family induces differentiation by silencing genes that maintain stemness and is repressed by the RNA-binding proteins LIN28A/B, which preserve stemness in normal embryonic and malignant cells. Here, we observed that LIN28A/B is undetectable in glioma stem cells (GSC) whereas let-7 and, paradoxically, their target genes are highly expressed. Using photoactivatable-ribonucloside-enchanced crosslinking and immunoprecipitation (PAR-CLIP), we show that insulin-like growth factor-2 mRNA-binding protein 2 (IMP2) protects let-7 target genes from silencing and provides a mechanistically distinct alternative to LIN28A/B toward both GSC and neural stem cell specification. Our observations define the RNA-binding repertoire of IMP2 and identify a mechanism by which it supports GSC maintenance.
2016-05-12 | GSE73846 | GEO
Project description:Different from most publication to date, in this study we find IMP1 and IMP2, two closely related RNA binding proteins, have different clients in MEF cells
2019-12-22 | GSE133242 | GEO