Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genetic mapping of potato cyst nematode (Globodera pallida) resistance in potato

ABSTRACT: Characterisation and mapping of a Globodera pallida resistance derived from the wild potato species Solanum spegazzinii

PROVIDER: PRJEB73769 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR12738213_1.fastq.gz	Fastqsanger.gz
	ERR12738213_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Potato cyst nematode repsonses in potato

Project description:Sets of seven 2-week old potato plants carrying the nematode resistance gene H1, grown from tuber ‘chits’ in sandy loam at a constant temperature of 20 ºC and a light cycle of 16 hour light/8 hour dark, were each inoculated in the roots evenly with 2000 juveniles of the virulent potato cyst nematode Globodera pallida, or with the avirulent G. rostochiensis pathotype Ro1, or with water. Plants were manually watered throughout the duration of the experiment. 5, 17 and 33 days after inoculation, the roots were carefully washed and root tissue samples were individually flash-frozen in liquid nitrogen and stored at –80 ºC. Total RNA isolations were performed using the Qiagen RNeasy kit. All samples were treated with DNase. The experiment was replicated twice. Keywords: Direct comparison

2007-09-01 | GSE8220 | GEO

DNAseq of nine Seresta-selected and unselected Globodera pallida populations

Project description:DNA sequencing of nine Globodera pallida populations selected either for four generations on the resistant potato variety Seresta (S4), or, as a control, for one generation on the susceptible variety Desiree (D). Seresta contains GpaV from Solanum vernei as a major resistance against G. pallida. These nine populations were obtained from Dutch potato fields, where they previous to the further selection already showed some degree of virulence on resistant potato varieties. The goal of the experiment was to identify polymorphic loci in the genome associated with the selection on Seresta.

2026-03-16 | E-MTAB-16285 | biostudies-arrayexpress

Selection for virulence in the potato cyst nematode Globodera pallida.

Project description:Selection for virulence against resistance in the potato cyst nematode Globodera pallida.

| PRJEB41175 | ENA

Globodera pallida transcriptomics

Project description:As part of the Globodera pallida (potato cyst nematode) genome project weare profiling the transcriptome of the parasite across its life cycle usingRNA-Seq. . This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2012-11-28 | E-ERAD-55 | biostudies-arrayexpress

Small RNA-seq on a time series of potato infected with Globodera pallida

Project description:We explored the sex determination of Globodera pallida. To do so, we grew cuttings of the potato cultivar Desiree on Gamborg B5 medium supplemented with either 1.5 g/L or 20 g/L sucrose. 14-day-old cuttings were inoculated with 100 G. pallida E400 Rookmaker juveniles. Infected root tissue was harvested at 1, 3, 6, and 9 dpi. Also, a subsample of the ppJ2 was kept for each batch. The experiment contains four time-separated batches. Small RNA sequencing was performed by BGI Hong Kong.

2025-11-05 | E-MTAB-15069 | biostudies-arrayexpress

Globodera pallida

Project description:Globodera pallida overview

| PRJNA714713 | ENA

Time-series RNA-seq on potato root tissue infected by two populations of Globodera pallida

Project description:In this experiment we follow the infection of potato by Globodera pallida populations AMPOP02 and Rookmaker. The goal of the experiment was twofold: (i) to follow the expression of effectors in the nematode, (ii) to follow the transcriptional response of the potato plant. In this experiment, we inoculated 14 day old potato cuttings of the variety Seresta with AMPOP02, Rookmaker, or a mock-infection. Root tissue was harvested at 1, 3, 6 and 9 days post infection. Also, a subsample of the pre-parasitic juveniles used as the inoculum was kept for each batch. The experiment contains four time-separated batches. RNA extraction was done in Wageningen. Sequencing was performed by BGI Hong Kong.

2025-08-05 | E-MTAB-15312 | biostudies-arrayexpress

Transcriptome sequencing on a time series of potato infected with Globodera pallida to explore the sex determination

Project description:We explored the sex determination of Globodera pallida. To do so, we grew cuttings of the potato cultivar Desiree on Gamborg B5 medium supplemented with either 1.5 g/L or 20 g/L sucrose. 14-day-old cuttings were inoculated with 100 G. pallida E400 Rookmaker juveniles. Infected root tissue was harvested at 1, 3, 6, and 9 dpi. Also, a subsample of the ppJ2 was kept for each batch. The experiment contains four time-separated batches. Transcriptome sequencing was performed by BGI Hong Kong. Two samples (B_4.2_1,5; B_4.3_1,5) were not taken along for sequencing because of a failed library prep.

2025-11-25 | E-MTAB-16117 | biostudies-arrayexpress

Gene expression in populations of the potato cyst nematode Globodera pallida in diapause or quiescence following hydration or exposure to tomato root diffusate

Project description:Plant-parasitic nematodes (PPN) need to be adapted to survive in the absence of a suitable host or in hostile environmental conditions. Various forms of developmental arrest (including desiccation, cryopreservation, hatching inhibition and dauer stages) are used by PPN in order to survive these conditions and spread to other areas. Potato cyst nematodes (PCN) (Globodera pallida and G. rostochiensis) are frequently in a dessicated state unhatched nematodes within the egg dispersal unit inside the cyst. Long term survival seems to be associated primarily with species that have a very restricted host range which requires surviving unhatched in the absence of the host for extended periods of time. This paper shows fundamental changes in the response of quiescent and diapaused eggs of G.pallida to hydration and following exposure to tomato root diffusate using microarray gene expression analysis from a broad set of genes. Surprisingly, many unique genes were activated in the population of diapaused eggs. Transport activity was activated in both quiescent and diapaused eggs; however, the transport function genes were very different between them. Hydrated quiescent and diapaused eggs were markedly different indicating differences in adaptation for long term survival.

2016-02-01 | E-MTAB-3824 | biostudies-arrayexpress

Globodera pallida

Project description:Globodera pallida Raw sequence reads

| PRJNA1167637 | ENA