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RNF20/RNF40 supports aggressive behavior in cervical cancer by regulating peroxisome-based anti-ferroptotic mechanism

ABSTRACT: RNF20/RNF40 supports aggressive behavior in cervical cancer by regulating peroxisome-based anti-ferroptotic mechanism

PROVIDER: PRJEB73996 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12757313.fastq.gz	Fastqsanger.gz
	ERR12757314.fastq.gz	Fastqsanger.gz
	ERR12757315.fastq.gz	Fastqsanger.gz
	ERR12757316.fastq.gz	Fastqsanger.gz
	ERR12757317.fastq.gz	Fastqsanger.gz

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RNF20/RNF40 supports aggressive behavior in cervical cancer by regulating peroxisome-based anti-ferroptotic mechanism

Project description:Cervical cancer treatments sometimes prove ineffective, indicating a need for personalized therapies. The present study demonstrate a strong correlation between high levels of the RNF20/RNF40 protein complex and aggressive cervical cancer. Our transcriptome analyzes uncovered a previously unknown role of this protein complex regulating peroxisomal pathway genes, which are crucial for lipid metabolism and the balance of reactive oxygen species. The loss of RNF20/RNF40 leads to reduced effectiveness of these genes, increasing lipid peroxidation and inducing a form of programmed cell death known as ferroptosis. These findings suggest that targeting the RNF20/RNF40 protein complex and its regulation capabilities could lead to new treatments for aggressive cervical cancer.

2025-03-09 | E-MTAB-13917 | biostudies-arrayexpress

Regulation of gene expression by WAC/RNF20/RNF40 in HEK293A cells

Project description:WAC is a known positive regulator of (macro)autophagy. WAC also forms a complex with RNF20/RNF40 to promote H2B monoubiquitination and hence to affect transcriptional regulation. This study addresses whether the WAC/RNF20/RNF40 complex regulates autophagy through effects on gene expression. WAC, RNF20 and RNF40 were knocked-down using pools of siRNAs in HEK293A cells. Each knockdown was in triplicate and the control was RISCfree siRNA. mRNA expression profiles were investigated using an Illumina HT12v4 Bead Array.

2015-12-17 | E-GEOD-66475 | biostudies-arrayexpress

Regulation of gene expression by WAC/RNF20/RNF40 in HEK293A cells

2015-12-17 | GSE66475 | GEO

mRNA-seq of intestinal epithelial cells isolated from Rnf20 and Rnf40 knockout mice

Project description:Intestinal epithelial cells (IECs) were isolated from the colon of Villin-CreERT2, Rnf20-flox and Rnf40-flox mice two weeks upon the Tamoxifen-induced, intestinal knockout of Rnf20 and Rnf40. RNA was isolated from snap-frozen IECs to perform mRNA-seq.

2021-06-16 | E-MTAB-9405 | biostudies-arrayexpress

H3K4me3 occupancy after Rnf20 and Rnf40 knockout in intestinal epithelial cells isolated from mice

Project description:Intestinal epithelial cells (IECs) were isolated from the colon of Villin-CreERT2, Rnf20-flox and Rnf40-flox mice two weeks upon the Tamoxifen-induced, intestinal knockout of Rnf20 and Rnf40. ChIP-seq for H3K4me3 was performed using snap-frozen IECs.

2021-06-16 | E-MTAB-9409 | biostudies-arrayexpress

The HDAC inhibitor Panobinostat (LBH589) exerts in vivo anti-leukaemic activity against in MLL-rearranged Acute Lymphoblastic Leukaemia and involves the RNF20/RNF40/WAC – H2B ubiquitination axis

Project description:We demonstrate the in vivo efficacy of the histone deacetylase inhibitor Panobinostat (LHB589) against MLL-rearranged ALL using xenograft mouse models of MLL-rearranged ALL cell lines and primary patient cells. Panobinostat monotherapy showed strong anti-leukaemic effects, extending survival and reducing overall disease burden. Comprehensive molecular analyses in vitro showed the anti-leukaemic activity in MLL-rearranged ALL to involve depletion of H2B ubiquitination via suppression of the RNF20/RNF40/WAC E3 ligase complex.

2017-07-12 | GSE78234 | GEO

mRNA-seq of intestinal epithelial cells isolated from Rnf20 and Rnf40 knockout mice

Project description:mRNA-seq of intestinal epithelial cells isolated from Rnf20 and Rnf40 knockout mice

| PRJEB39653 | ENA

RNF40 exerts stage-dependent functions in differentiating osteoblasts and is essential for bone cell crosstalk [RNA-seq]

Project description:In this study, we focused on elucidating the role of Ring Finger Protein 40 (RNF40) in bone development by using a conditional knockout mouse approach during different stages of osteoblast (OB) differentiation and maturation. RNF40 forms an obligate E3 ubiquitin ligase complex together with RNF20 and mediates the monoubiquitination of lysine 120 of histone H2B (H2BK120ub1). We provide evidence that RNF40 regulates OB differentiation in a stage-dependent manner. Additionally, we show that RNF40 is required for bone cell crosstalk whereby loss of RNF40 leads to a reduction in osteoclast numbers and function through modulation of vitamin D receptor (VDR)-induced Rankl expression in OBs. Taken together, these data imply an important role of RNF40-mediated H2Bub1 in bone formation and remodeling and provide a basis for further investigation of its anti-resorptive potential for the treatment of conditions such as osteoporosis or cancer-associated osteolysis. In this study we show that RNF40 is required in earlier stages of osteoblast differentiation and is involved in bone cell crosstalk by regulating vitamin D induced Rankl expression

2021-02-24 | GSE135628 | GEO

RNF40 exerts stage-dependent functions in differentiating osteoblasts and is essential for bone cell crosstalk [ChIP-seq]

2021-02-24 | GSE135627 | GEO

H3K4me3 occupancy after Rnf20 and Rnf40 knockout in intestinal epithelial cells isolated from mice

Project description:H3K4me3 occupancy after Rnf20 and Rnf40 knockout in intestinal epithelial cells isolated from mice

| PRJEB39656 | ENA