Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Quantitative measurement of phenotype dynamics during cancer drug resistance evolution using genetic barcoding

ABSTRACT: Quantitative measurement of phenotype dynamics during cancer drug resistance evolution using genetic barcoding

PROVIDER: PRJEB74046 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR12766011_1.fastq.gz	Fastqsanger.gz
	ERR12766011_2.fastq.gz	Fastqsanger.gz
	ERR12766012_1.fastq.gz	Fastqsanger.gz
	ERR12766012_2.fastq.gz	Fastqsanger.gz
	ERR12766512_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 230

Similar Datasets

SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression.

Project description:Single-cell mRNA sequencing (RNA-seq) methods have undergone rapid development in recent years, and transcriptome analysis of relevant cell populations at single-cell resolution has become a key research area of biomedical sciences. We here present single-cell mRNA 3-prime end sequencing (SC3-seq), a practical methodology based on PCR amplification followed by 3-prime-end enrichment for highly quantitative, parallel and cost-effective measurement of gene expression in single cells. The SC3-seq allows excellent quantitative measurement of mRNAs ranging from the 10,000-cell to 1-cell level, and accordingly, allows an accurate estimate of the transcript levels by a regression of the read counts of spike-in RNAs with defined copy numbers. The SC3-seq has clear advantages over other typical single-cell RNA-seq methodologies for the quantitative measurement of transcript levels and at a sequence depth required for the saturation of transcript detection. The SC3-seq distinguishes four distinct cell types in the peri-implantation mouse blastocysts. Furthermore, the SC3-seq reveals the heterogeneity in human-induced pluripotent stem cells (hiPSCs) cultured under on-feeder as well as feeder-free conditions, demonstrating a more homogeneous property of the feeder-free hiPSCs. We propose that SC3-seq might be used as a powerful strategy for single-cell transcriptome analysis in a broad range of investigations in biomedical sciences.

2015-03-20 | GSE63266 | GEO

Highly quantitative measurement of differential protein-genome binding with PerCell chromatin sequencing

Project description:Highly quantitative measurement of differential protein-genome binding with PerCell chromatin sequencing

| PRJNA1134248 | ENA

A proteomic barcoding platform for macromolecular screening and delivery

Project description:To address the delivery barriers of macromolecular therapeutics, we sought to develop high-throughput peptide barcoding platform as a homogeneous method for macromolecular screening. Implemented in open-source algorithms, BarcodeBabel and PeptideBabel, this approach permits the design of libraries of peptide barcodes and novel penetration sequences optimized for analysis by quantitative mass spectrometry.

2025-05-06 | PXD048412 | Pride

High-efficiency RNA cloning enables quantitative measurement of miRNA expression by deep sequencing

Project description:High-efficiency RNA cloning enables quantitative measurement of miRNA expression by deep sequencing

| PRJNA208235 | ENA

S100A4 mRNA-protein relationship uncovered by measurement noise reduction

Project description:Intrinsic biological fluctuation and/or measurement error can obscure the association of gene expression patterns between RNA and protein levels. Appropriate normalisation of reverse-transcription quantitative PCR (RT-qPCR) data can reduce technical noise in transcript measurement, thus uncovering such relationships. The accuracy of gene expression measurement is often challenged in the context of cancer due to its genetic instability and ‘splicing weakness’. Here we sequenced the poly(A) cancer transcriptome of canine osteosarcoma using mRNA-Seq. Expressed sequences were resolved at the level of two consecutive exons to enable the design of exon-border spanning RT-qPCR assays and ranked for stability based on the coefficient of variation (CV). Using the same template type for RT-qPCR validation, i.e. poly(A) RNA, avoided skewing of stability assessment by circular RNAs (circRNAs) and/or rRNA deregulation. The strength of the relationship between mRNA expression of the tumour marker S100A4 and its proportion score of quantitative immunochemistry (qIHC) was introduced as an experimental read-out to fine-tune the normalisation choice. Together with the essential logit transformation of qIHC scores, this approach reduced the noise of measurement as demonstrated by uncovering a highly significant, strong association between mRNA and protein expressions of S100A4 (Spearman's coefficient r = 0.72 (p = 0.006)).

2020-03-26 | GSE147550 | GEO

SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression.

Project description:SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression.

| PRJNA267121 | ENA

MinION barcoding

Project description:MinION DNA barcoding wildlife samples

| PRJEB36404 | ENA

NK cell cytotoxicity shapes the clonal evolution of B cell leukemia [DNA barcoding]

Project description:The term cancer immunoediting describes the dual role by which the immune system can suppress and promote tumour growth and is divided into three phases: elimination, equilibrium and escape. The role of NK cells has mainly been attributed to the elimination phase. Here we show that NK cells play a role in all three phases of cancer immunoediting. Extended co-culturing of DNA barcoded mouse BCR/ABLp185+ B acute lymphoblastic leukaemia cells with NK cells allowed for a quantitative measure of NK cell-mediated immunoediting. Whereas most tumour cell clones were efficiently eliminated by NK cells, a certain fraction of tumour cells harboured an intrinsic primary resistance. Furthermore, DNA barcoding revealed tumour cell clones with secondary resistance, which stochastically acquired resistance to NK cells. NK cell cytotoxicity put a selective pressure on B-ALL cells inducing primary and secondary resistance, while resistant tumour cells were characterised by a full-blown IFN-g signature. Besides well-known regulators of immune evasion, our analysis of NK resistant tumour cells revealed the upregulation of novel genes, including Ly6a, which we found to drive NK cell resistance in leukaemic cells. We further translated our findings to the human system and showed that high LY6E expression on tumour cells impaired the physical interaction with NK cells and led to worse prognosis in leukaemia. Our results demonstrate that tumour cells are actively edited by NK cells during the equilibrium phase and use different avenues to escape NK cell-mediated eradication.

2024-11-26 | GSE278011 | GEO

Quantitative measurement of full-length transcript isoforms reveals precise regulation of splicing in human heart cells

Project description:Quantitative measurement of full-length transcript isoforms reveals precise regulation of splicing in human heart cells

| PRJEB29366 | ENA

COL1A2 Barcoding: Bone Species Identification via Shotgun Proteomics

Project description:Species identification of fragmentary bones remains a challenging task in archeology and forensics. A species identification method for such fragmentary bones that has recently attracted interest is the use of bone collagen proteins. We developed a method similar to DNA barcoding that reads collagen protein sequences in bone and automatically determines the species by performing sequence database searches. We tested our method using bone samples from 30 vertebrate species ranging from mammals to fish.

2023-12-14 | PXD045402 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data