Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of the Sox17∆50/GFP mutation on SOX17 function in mouse embryonic liver development

ABSTRACT: Effects of the Sox17∆50/GFP mutation on SOX17 function in mouse embryonic liver development

PROVIDER: PRJEB74458 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR12815675_1.fastq.gz	Fastqsanger.gz
	ERR12815675_2.fastq.gz	Fastqsanger.gz
	ERR12815676_1.fastq.gz	Fastqsanger.gz
	ERR12815676_2.fastq.gz	Fastqsanger.gz
	ERR12815677_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Effects of the Sox17∆50/GFP mutation on SOX17 function in mouse embryonic liver development

Project description:To determine the role of specific cis-regulatory elements within the Sox17 endoderm-preferential TSS2 promoter, we generated Sox17∆50 mutant animals, crossed them to a Sox17GFPCre (a Sox17 null allele), and surveyed how this mutation affected Sox17 expression. Livers were isolated from E12.5 Sox17∆50/GFPCRe compound heterozygous and Sox17+/+ embryos (n = 4 of each genotype), RNA isolated, and bulk RNA-Seq performed.

2024-10-31 | E-MTAB-13983 | biostudies-arrayexpress

Effects of the Sox17 ∆50 mutation on SOX17 function in mouse embryonic liver development

2024-10-31 | E-MTAB-13982 | biostudies-arrayexpress

Effects of the Sox17∆50 mutation on SOX17 function in mouse embryonic biliary development

Project description:To determine the role of specific cis-regulatory elements within the Sox17 endoderm-preferential TSS2 promoter, we generated Sox17∆50 mutant animals and surveyed how this mutation altered Sox17 expression. EPCAM+/CXCR4+ endodermal cells were isolated from E10.5 Sox17∆50/∆50 and Sox17+/+ embryos (n = 1 of each genotype). Posterior foregut and midgut regions of embryos were dissected by removal of embryonic head, heart, tail, limb buds, posterior and dorsal body trunk. Sox17+/+ and Sox17Δ50/Δ50 cell samples were labelled with barcodes from 3'CellPlex Kit (10X Genomics) and subsequently pooled together for single isolation, library preparation and sequencing.

2024-10-31 | E-MTAB-14000 | biostudies-arrayexpress

Effects of the Sox17 ∆50 mutation on SOX17 function in mouse embryonic liver development

Project description:Effects of the Sox17 ∆50 mutation on SOX17 function in mouse embryonic liver development

| PRJEB74456 | ENA

Effects of the Sox17∆50 mutation on SOX17 function in mouse embryonic biliary development

Project description:Effects of the Sox17∆50 mutation on SOX17 function in mouse embryonic biliary development

| PRJEB74552 | ENA

Effects of the Sox17mut5 mutation on SOX17 function in mouse embryonic gallbladder development

Project description:To determine the role of specific cis-regulatory elements within the Sox17 endoderm-preferential TSS2 promoter, we generated Sox17mut5 mutant animals and surveyed how this mutation altered Sox17 expression. Gallbladders were isolated from P21 Sox17mut5/mut5 and Sox17+/+ (n = 4 of each genotype), RNA isolated, and bulk RNA-Seq performed.

2024-10-31 | E-MTAB-13978 | biostudies-arrayexpress

Gene expression data of Sox17-GFP(+) mouse definitive endoderm before and after expansion on mesenchyme.

2011-01-12 | GSE25337 | GEO

Effects of the Sox17mut5 mutation on SOX17 function in mouse embryonic gallbladder development

Project description:Effects of the Sox17mut5 mutation on SOX17 function in mouse embryonic gallbladder development

| PRJEB74445 | ENA

Gene expression data of Sox17-GFP(+) mouse definitive endoderm before and after expansion on mesenchyme.

Project description:Mouse embryonic stem cells containing a Sox17-GFP construct were differentiated using growth factors (Activin A and Wnt3A) to definitive endoderm. Sox17-GFP(+) cells were sorted using fluorescence activated cell sorting and either used for total RNA harvest OR continued in culture in the presence of primary pancreatic mesenchymal cell lines. At the end of 6 serial passages on mesenchyme, the Sox17-GFP(+) cells were again sorted and the RNA was harvested for arrays. Samples were prepared as described in summary, with technical duplicates for each of the following 3 categories: 1. Unpassaged (P0) endoderm, 2. Endoderm passaged 6 times (P6) on mesenchyme 1, and 3. Endoderm passaged 6 times (P6) on mesenchyme 2.

2011-01-12 | E-GEOD-25337 | biostudies-arrayexpress

Whole transcriptome analysis of Sox17.Epi and Endo cells in mouse embryos

Project description:This experiment has been done to understand the dynamics of gene expression in Sox17.Epi and Endo cells using multiplex RNA-Seq technology. Sox17.Epi (Sox17^GFP+EpCAM+) and Sox17.Endo (Sox17^GFP+EpCAM-) cells were isolated by FACS from E9.5 mouse embryos. The spatial comparison between Sox17.Epi versus Sox17.Endo provides insight into which genes are differentially of specifically expressed in two populations.

2012-07-20 | E-MTAB-970 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data