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Transcriptional changes associated with miR-6762-5p expression in HCT116 cells

ABSTRACT: Transcriptional changes associated with miR-6762-5p expression in HCT116 cells

PROVIDER: PRJEB76163 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR13172982_1.fastq.gz	Fastqsanger.gz
	ERR13172982_2.fastq.gz	Fastqsanger.gz
	ERR13172983_1.fastq.gz	Fastqsanger.gz
	ERR13172983_2.fastq.gz	Fastqsanger.gz
	ERR13172984_1.fastq.gz	Fastqsanger.gz

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Transcriptional changes associated with miR-6762-5p expression in HCT116 cells

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Project description:Post-transcriptional regulation of gene expression by miRNAs likely makes significant contributions to mRNA abundance at the embryo-maternal interface. In this study, we investigated how miR-26a-5p and miR-125b-5p contribute to molecular changes occurring in the uterine luminal epithelium, which serves as the first site of signal exchange between the mother and developing embryo. To measure de novo protein synthesis after miRNA delivery to primary uterine luminal epithelial cells, we employed pulsed stable isotope labeling by amino acids (pSILAC). We found that both miRNAs alter the proteome of luminal epithelial cells, impacting numerous cellular functions, immune responses, as well as intracellular and second messenger signaling pathways. Additionally, we identified several features of miRNA-mRNA interactions that may influence the targeting efficiency of miR-26a-5p and miR-125b-5p. Overall, our study suggests a complex interaction of miR-26a-5p and miR-125b-5p with their respective targets. However, both appear to cooperatively function in modulating the cellular environment of the luminal epithelium, facilitating the morphological and molecular changes that occur during the intensive communication between the embryo and uterus at pregnancy.

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