Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of Raji cells expressing either IL-13Rα1 or IL-13Rα1-LOR1a and treated with IL-13 or not

ABSTRACT: RNA-seq of Raji cells expressing either IL-13Rα1 or IL-13Rα1-LOR1a and treated with IL-13 or not

PROVIDER: PRJEB76782 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR13301203_1.fastq.gz	Fastqsanger.gz
	ERR13301203_2.fastq.gz	Fastqsanger.gz
	ERR13301204_1.fastq.gz	Fastqsanger.gz
	ERR13301204_2.fastq.gz	Fastqsanger.gz
	ERR13301205_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 72

Similar Datasets

RNA-seq of Raji cells expressing either IL-13Rα1 or IL-13Rα1-LOR1a and treated with IL-13 or not

Project description:Raji cells were transduced to express either that canonical IL-13Rα1 subunit of the shared type II receptor for IL-4 and IL-13 or a newly discovered, termed IL-13Rα1-LOR1a. These cells were treated with IL-13 and compared with untreated control cells, as well as with treated and untreated untransduced Raji cells, with the aim of assessing the potential of the IL-13Rα1-LOR1a isoform to transmit IL-13 signals. Each condition was tested in triplicate.

2025-04-18 | E-MTAB-14186 | biostudies-arrayexpress

IMR90 lung fibroblasts treated with IL-4 or IL-13

Project description:IL-4 and IL-13 are cytokines involved in type 2 immune responses involved in atopic asthma and other diseases. They have a partially overlapping set of cognate receptors whose roles are incompletely understood. Here we explore the effects of these cytokines on lung fibroblasts in order to assess the global similarities and contrasts in the genes whose expression their ligation modulates. IMR-90 lung fibroblast cells were cultured on matrigel or plastic and treated for 24 hours with IL-4 (10 ng/ml), IL-13 (10ng/ml), or TNFa (10 ng/ml) before harvest.

2013-12-31 | E-GEOD-47542 | biostudies-arrayexpress

Myeloid cells or lung fibroblasts treated with IL-4 or IL-13

Project description:IL-4 and IL-13 are cytokines involved in type 2 immune responses involved in atopic asthma and other diseases. They have a partially overlapping set of cognate receptors whose roles are incompletely understood. Here we explore the effects of these cytokines on various cells related to human lung disease in order to assess the global similarities and contrasts in the genes whose expression their ligation modulates. Monocytes, monocyte-differentiated (6 days, then 24h rest) macrophages, or normal primary lung fibroblasts were cultured on matrigel and treated for 6 or 24 hours with IL-4 (10 ng/ml), IL-13 (10ng/ml), IL-10 (20 ng/ml), TGFb (10 ng/ml), or dexamethasone (0.5 uM) before harvest.

2013-12-31 | E-GEOD-47538 | biostudies-arrayexpress

IL-13 involvement in eosinophilic esophagitis: transcriptome analysis

Project description:3 eosinophilic esophagitis biopsies, cultured and stimulated with IL-13 : each of them was either left unstimulated or stimulated (100ng for 48h) We used microarray to uncover the IL-13-induced genes in esophageal epithelial cells of the esophagus Keywords: treated vs non treated

2007-12-21 | GSE8853 | GEO

Human airway epithelial cells co-treated with IL-4 and IL-13 for seven days.

Project description:Nasal basal epithelial cells from 6 healthy adult donors were grown in air-liquid interface (ALI) cultures for fourteen days. Cells were then treated with 10 ng/mL of IL-4 and 10 ng/mL of IL-13 for seven additional days. Next, cell lysates were used for low-input RNA-seq library preparation (Smart-seq2) and sequencing.

2024-05-29 | GSE268072 | GEO

Effect of bryostatin-1 on gene expression profile of Raji cells

Project description:To investigate bryostain's effect on Raji , we performed a whole-transcriptome RNA sequencing experiment for Raji cells treated with Bryostatin and established differntial expression profile comparing to raji treated with DMSO.

2022-08-19 | GSE211351 | GEO

IMR90 lung fibroblasts treated with IL-4 or IL-13

2013-12-31 | GSE47542 | GEO

Transcription profiling of human Burkitts lymphoma cells line Raji expressing the TM4SF member CD9

Project description:Expression of the TM4SF member CD9 on the human Burkitts lymphoma cell line Raji induced increased cell proliferation, motilty and adhesion to fibronectin. CD9 promoted increases in Raji cell proliferation was dependent upon histone deacetyalase (HDAC) activity as treatment with HDAC inhibitors trichostain A or cucurmin attenuated CD9 mediated increases in Raji cell proliferation. Gene expression of Raji cells stably expressing human CD9 via transfection with expression vector PRVCMVCD9 was compared with corresponding Mock transfected cell by microarray analysis using the Affymetrix U133 2.0 platform. Experiment Overall Design: Transfected Raji cells that had been in cultured in RPMI, 10% FBS in the presence of 1mg/ml G418 were harvested during exponential growth and RNA was extracted using TRiReagent according to manufacturer's protocol (Sigma).

2009-10-27 | E-GEOD-13210 | biostudies-arrayexpress

PGA Murine IL-13 Asthma

Project description:The IL-13 is a central mediator of allergic asthma. This project investigates the mechanisms by which IL-13 elicits the symptoms of asthma. Keywords: other

2004-04-14 | GSE1301 | GEO

Whole Lung Proteome in N. brasiliensis Infection in IL-13 deficient mice.

Project description:IL-13 plays a key role during protective type 2 immune responses at mucosal sites, such as during infection with nematodes. However, dysregulation of IL-13 can also contribute to the pathogenesis of allergic and fibrotic diseases. Matrix remodelling is an important component of repair processes in the lung but is also a hallmark of chronic diseases such as asthma. Since IL-13 shares receptors and signalling pathways with IL-4, disentangling the relative contributions of these two type 2 cytokines has been challenging. Additionally, little is known about the singular role of IL-13 in more acute settings of tissue injury/repair and whether IL-13 regulates remodelling of the extracellular matrix following tissue injury. In this study, we used Nippostrongylus brasiliensis infection as model of acute lung tissue damage and repair by comparing responses between WT and IL-13-deficient mice, in which IL-4 signalling is intact.

2021-06-11 | PXD021853 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data