Project description:YTHDC1 is one of m6A reader proteins exerting multifaceted effects on determining the fate of m6A RNA within the nucleus and highly expressed in liver cancer cells.To understand how YTHDC1 contributes to liver cancer progression, we conducted MeRIP-seq assay in HepG2 cells and aimed to identified YTHDC1 target transcripts.
Project description:YTHDC1 is one of m6A reader proteins exerting multifaceted effects on determining the fate of m6A RNA within the nucleus.To understand how YTHDC1 contributes to liver cancer progression, we conducted RNA-seq assay in shCtrl and shDC1 HepG2 cells and aimed to identified YTHDC1-responsive genes.
Project description:Identification of liver transcription factors binding sites by ChIP-chip using HepG2 cells. Inference of binding sites at base pair resolution was achieved by using bioinformatic tools on the generated data sets.
Project description:Berberine, an isoquinoline alkaloid isolated from many medicinal herbs such as Coptis chinensis, has a wide range of pharmacological effects. Here, we used gene expression microarray to analyze gene expression profiles of HepG2 human hepatoma cell line after berberine chloride treatment or 0.08% DMSO as control. Comparing gene expression profiles of 40 M-BM-5M-BM--M berberine-treated HepG2 human hepatoma cell line to those of control cells sampled after 4 hours treatment. A 50 mM stock solution of Berberine chloride was prepared in DMSO. Cells were treated with 40 M-BM-5M-BM--M berberine chloride or 0.08% DMSO as control.
Project description:mRNA-seq and high throughput MeRIP-seq methods with bioinformatics analysis demonstrated a possible mechanism for the inhibition of cell cycle of hepatoma HepG2 cells induced by toosendanin.