1,25-dihydroxyvitamin D3 augments low-dose PMA-based monocyte-to-macrophage differentiation in THP-1 cells
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB76891 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:THP-1 cells PMA-treated RNA-seq
| PRJNA632845 | ENA
Project description:Retinoid X receptors (RXRs) act as obligate dimerisation partners for multiple nuclear receptors (NRs), each with distinct regulatory functions. In this study, human PMA-differentiated THP-1 (PMA-THP-1) cells were treated with single or combined ligands for RXR or six partners for 2 hours, and the genomic binding landscape of RXR was investigated using ChIP-seq. RXR genomic binding regions were also mapped following 1 hour of treatment with vehicle or 1,25-vitD using ChIP-seq. In addition, histone H3 lysine 27 acetylation (H3K27ac) was mapped following 6 hours of treatment with vehicle or 1,25-vitD using ChIP-seq. In parallel, RNA-seq was performed on PMA-THP-1 cells following 6 hours of treatment with vehicle, 1,25-vitD or combined ligands to identify and compare the regulated gene sets.
2026-05-14 | GSE315572 | GEO
Project description:Retinoid X receptors (RXRs) act as obligate dimerisation partners for multiple nuclear receptors (NRs), each with distinct regulatory functions. In this study, human PMA-differentiated THP-1 (PMA-THP-1) cells were treated with single or combined ligands for RXR or six partners for 2 hours, and the genomic binding landscape of RXR was investigated using ChIP-seq. RXR genomic binding regions were also mapped following 1 hour of treatment with vehicle or 1,25-vitD using ChIP-seq. In addition, histone H3 lysine 27 acetylation (H3K27ac) was mapped following 6 hours of treatment with vehicle or 1,25-vitD using ChIP-seq. In parallel, RNA-seq was performed on PMA-THP-1 cells following 6 hours of treatment with vehicle, 1,25-vitD or combined ligands to identify and compare the regulated gene sets.
2026-05-14 | GSE315571 | GEO
Project description:Myeloid leukemia cell lines HL60, THP-1, and U937 undergo macrophage-like differentiation after treatment with phorbol ester. To explore genes whose exon usage was altered during macrophage differentiation, we compared exome of PMA-treated (differentated) and vehicle-treated (undifferentiated) myeloid cell lines. HL60, THP-1, and U937 cells were treated with either phorbol 12-myristate 13-acetate (PMA,30nM) or its vehicle (DMSO) for 3 days, and subjected to exome analysis using Affymetrix human exon 1.0ST arrays.
2013-09-25 | E-GEOD-20567 | biostudies-arrayexpress
Project description: Not available
| S-EPMC10544208 | biostudies-literature
Project description:We analysed the capacity of THP-1 cells (differentiated to macrophagoid cells) to recognize RNA sequences via pattern recognition receptors in vitro. Gene expression was analysed by RNA-Microarray. Cytokine production was analysed by ELISA assays. We used microarrays to investigate differential gene expression in THP-1 cell line undifferentiated in comparison with 3 days or 8 days differentiated with phorbol myristate acetate (PMA). Microarray analysis revealed differential gene expression patterns of THP-1 when differentiated. THP-1 cells, undifferentiated, 3 days PMA-differentiated and 8 days PMA-differentiated
2015-11-09 | E-GEOD-67264 | biostudies-arrayexpress
Project description:Myeloid leukemia cell lines HL60, THP-1, and U937 undergo macrophage-like differentiation after treatment with phorbol ester. To explore genes whose exon usage was altered during macrophage differentiation, we compared exome of PMA-treated (differentated) and vehicle-treated (undifferentiated) myeloid cell lines.
2013-09-25 | GSE20567 | GEO
Project description:RNA-Seq was carried out in order to obtain the expression profile of lipopolysaccharide (LPS)-induced transcriptome changes in PMA-differentiated human THP-1 cell line.
2021-10-17 | E-MTAB-10513 | biostudies-arrayexpress
Project description:Macrophages play a key role in both innate and adaptive immunity, but our knowledge on the changes in transcription regulation that occurs during their differentiation from monocytes is still limited. In this study, we used a meta-analysis followed by a systems biology approach for the identification of differentially expressed genes between monocytes and macrophages and possible regulators of these changes in transcription. Based on the pattern of gene expression change, transcription regulator analysis predicted a decrease in Enhancer of Zeste homolog 2 (EZH2), a histone 3 lysine 27 methyl transferase, activity after differentiation of monocytes into macrophages. This inhibition was validated by a significant decrease in trimethylated H3K27 during differentiation of both human primary monocytes into macrophages and the THP-1 cell line into macrophage-like cells. Overexpressing EZH2 during differentiation of monocytes and THP-1 cells obstructs cellular adhesion, thus preventing the first step in differentiation. Another facet of macrophage differentiation is the cessation of proliferation, and inhibition of EZH2 by the small molecule inhibitor GSK126 in THP-1 cells indeed impedes proliferation. This study shows an important part for epigenetic changes during monocyte differentiation. It highlights the role of EZH2 activity behind the changes needed in adhesion and proliferation mechanisms for macrophage formation. THP-1s were differentiated into macrophage like cells by PMA stimulation.
2013-12-28 | E-GEOD-53691 | biostudies-arrayexpress
Project description:Recently, it has been reported that 25(OH)D3 (25D3) has physiological bioactivity in certain tissues derived from the Cyp27b1 knockout mice. To investigate 25D3 function in the kidney as an informational crossroad of various calciotropic substances, we employed CRISPR-Cas9 system to knock out the Cyp27b1 gene in the mouse renal tubular cell line, mDCT cells. Unlike the previously reported mice targeted to the Cyp27b1 gene systemically, Cyp27b1 knockout mDCT cells did not produce any measurable 1a,25(OH)2D3 (1,25D3) after 25D3 administration. As was seen in the treatment with 10-8 M and higher dose of 1,25D3, we found that 10-7 M of 25D3 could translocate VDR into the nucleus and promoted expression of the representative 1,25D3-responsive Cyp24a1 gene in the Cyp27b1 knockout mDCT cells. The exhaustive target gene profiles of 25D3 showed results closely mimicking those of 1,25D3. Subsequently, we confirmed that 25D3 induced the expression of a calcium reabsorption-related gene, Calbindin-D9K gene, in a similar way to 1,25D3. As another example among others, we found that both 1,25D3 and 25D3 induced the expression of Megalin gene. Our ChIP assay identified that two VDRE sites at the upstream region of the Megalin gene contributed to such gene activation. Together, we surmise that the ability to stimulate VDR target genes may provide a novel perspective with 25D3 contribution in certain tissues.
2019-08-30 | GSE136594 | GEO