RNASeq analysis of brown bear (Ursus arctos) skin tissue during active state and hibernation
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB80554 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptomic analysis of the brown bear (Ursus arctos) tissues during both active and inactive phases has unveiled significant differential gene expression, with thousands of genes exhibiting altered activity in all studied tissues (muscle, liver, adipose...). Nevertheless, transcriptional analyses of hibernating animal tissues are limited to organs that are important in metabolism. No information is available about the variations of gene expression in tissues that may have a lower impact on metabolism, like the skin, which is mainly a support tissue and a mechanical barrier against the external environment. That experiment brings information about the gene expression variations in the bear skin during hibernation.
2025-09-05 | E-MTAB-14479 | biostudies-arrayexpress
Project description:During hibernation, the physiology of bears is characterized by strong metabolic depression. In skeletal muscles, lipids are used as the main energy fuels although their oxidation rate is reduced, and glycolysis appears maintained likely feeding the Cori cycle. For a better knowledge on proteome remodelling during hibernation, this study analysed the bear (Ursus arctos) skeletal muscle proteome using shotgun proteomics to compare hibernation to the summer-active period.
2026-03-02 | PXD060503 | Pride
Project description:Brown bears (Ursus arctos) hibernate for 5-7 months without eating, drinking, urinating and defecating at a metabolic rate of only 25% of the summer activity rate. Nonetheless, they emerge healthy and alert in spring. We analyzed the physiological adaptations for hibernation by comparing the proteome, metabolome, and hematologic features of blood from hibernating and active free-ranging sub-adult brown bears with a focus on conservation of health and energy. We found that the concentrations of most plasma proteins and white blood cell types decreased during hibernation. Strikingly, antimicrobial defense proteins increased in concentration. Central functions in hibernation involving the coagulation response and protease inhibition, as well as lipid transport and metabolism, were upheld by increased levels of very few key or broad-specificity proteins. A dramatic 45-fold increase in sex-hormone-binding-globulin SHBG levels during hibernation draws, for the first time, attention to its significant but unknown role in maintaining hibernation physiology. We propose that energy for the costly protein synthesis is reduced by three mechanisms, (i) dehydration, which increases protein concentration without de novo synthesis; (ii) reduced protein degradation rates due to a 6°C reduction in body temperature, decreased protease activity, and increased plasma viscosity; and (iii) a marked redistribution of energy resources only increasing de novo synthesis of few key proteins. This comprehensive global data identified novel biochemical strategies for bear adaptations to the extreme condition of hibernation, and have implications for our understanding of physiology in general.
2016-09-27 | PXD003946 | Pride
Project description:Male and female 2- and 3-years old brown bear from the region of Tackasen (Sweden) were captured in summer and winter. Muscle biopsies from vastus lateralis were collected in february (hibernation state) and june (active period). Total RNA were extracted from muscle tissue and full transcriptome analysis (RNA Seq) were performed. Statistical analysis were performed to winter versus summer comparison from matched paired samples
2021-09-01 | GSE144856 | GEO
Project description:During hibernation, the physiology of bears is characterized by strong metabolic depression. In skeletal muscles, lipids are used as the main energy fuels although their oxidation rate is reduced, and glycolysis appears maintained likely feeding the Cori cycle. Mitochondria no doubt play an essential role in such an adaptation. However, neither their activity nor their protein content had been analysed earlier. To fill this gap, this study analysed the bear (Ursus arctos) skeletal muscle mitochondrial fraction using shotgun proteomics to compare hibernation to the summer-active period.
2026-03-02 | PXD036916 | Pride
Project description:North American brown bear (Ursus arctos horribilis) genome sequencing and assembly
| PRJNA487641 | ENA