Bacteriophage genome submission
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ABSTRACT:
PROVIDER: PRJEB91637 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Escherichia coli and Staphylococcus aureus are two common pathogenic microorganisms that cause mastitis in dairy cows. They can cause clinical mastitis and subclinical mastitis. In recent studies, lncRNAs have been found to play an important role in the immune responses triggered by microbial inducers. However, the actions of lncRNAs in bovine mastitis remain unclear. The purpose of this study was to explore the lncRNA profile on mastitis.
2021-08-06 | GSE181464 | GEO
Project description:Staphylococcus aureus causes disease in humans and a wide array of animals. Of note, S. aureus mastitis of ruminants, including cows, sheep and goats, results in major economic losses worldwide. Extensive variation in genome content exists among S. aureus pathogenic clones. However, the genomic variation among S. aureus strains infecting different animal species has not been well examined. To investigate variation in the genome content of human and ruminant S. aureus we carried out whole genome PCR scanning (WGPS), comparative genomic hybridizations (CGH), and directed DNA sequence analysis of strains of human, bovine, ovine, and caprine origin. Extensive variation in genome content was discovered including host- and ruminant-specific genetic loci. Ovine and caprine strains were genetically allied whereas bovine strains were heterogenous in gene content. As expected, mobile genetic elements such as pathogenicity islands and bacteriophages contributed to the variation in genome content between strains. However, remarkably, most host-specific differences were restricted to regions of the conserved core genome, which contained allelic variation in genes encoding proteins of known and unknown function. Many of these proteins are predicted to be exported and could play a role in host-pathogen interactions. These data suggest that diversification of the core genome may be more important than acquisition of novel genes for S. aureus host-adaptation. The host-specific determinants identified by the whole-genome approaches adopted in the current study represent excellent targets for studies of the evolution and molecular basis of S. aureus host specificity. Keywords: Strain vs strain eleven strains of Sa were compared at the DNA level in triplicate.
2008-11-18 | E-GEOD-10187 | biostudies-arrayexpress
Project description:Mastitis is a common disease in dairy cows and brings massive losses to the dairy industry. m6A is a type of modification strongly associated with many diseases. However, the role of m6A in mastitis caused by Staphylococcus aureus and Escherichia coli has not been investigated.We used MeRIP-seq technology to sequence the bovine mammary epithelial cells (MAC-T) infected with inactivated S. aureus/E. coli for 24 h.
2020-11-09 | GSE161050 | GEO
Project description:In vitro challenge of bovine macrophages (derived from blood monocytes)with live bacteria of a Staphylococcus aureus strain isolated from a clinical mastitis case.
2012-01-01 | E-TABM-1133 | biostudies-arrayexpress
Project description:In the face of escalating challenges posed by infections with multiple drug-resistant (MDR) pathogens, the use of bacteriophages in therapeutic applications has emerged as a critically relevant and often sole alternative to traditional antibiotics. The most promising results of phage therapy have been demonstrated in the case of Staphylococcus aureus. It should be noted that the S. aureus population is characterized by a high degree of clonality. Strains belonging to sequence type 239 are among the main epidemic strains of methicillin-resistant S. aureus, identified worldwide. Many ST239 strains are characterized by MDR and resistance to virulent bacteriophages, and can become a serious problem in medicine. The purpose of this study was to investigate the resistance of clinical strains of S. aureus SA191REV of ST239 to bacteriophages of the Herelleviridae family using systems biology methods, starting from microbiological data on the interaction of strains with bacteriophages to transcriptomic and proteomic analysis. As results, the main changes in the cells of the resistant strain caused by phage infection are related to the energy metabolism in the cell. Significant changes were found in the functioning of the tricarboxylic acid cycle, glycolytic process, and glucose metabolic process. The most likely mechanism of resistance in the case of SA191REV strains is the premature death of infected cells due to the type I toxin-antitoxin system, as well as the hyperexpression of the lrgA and lrgB genes.
2025-05-06 | PXD047971 | Pride
Project description:Staphylococcus aureus causes disease in humans and a wide array of animals. Of note, S. aureus mastitis of ruminants, including cows, sheep and goats, results in major economic losses worldwide. Extensive variation in genome content exists among S. aureus pathogenic clones. However, the genomic variation among S. aureus strains infecting different animal species has not been well examined. To investigate variation in the genome content of human and ruminant S. aureus we carried out whole genome PCR scanning (WGPS), comparative genomic hybridizations (CGH), and directed DNA sequence analysis of strains of human, bovine, ovine, and caprine origin. Extensive variation in genome content was discovered including host- and ruminant-specific genetic loci. Ovine and caprine strains were genetically allied whereas bovine strains were heterogenous in gene content. As expected, mobile genetic elements such as pathogenicity islands and bacteriophages contributed to the variation in genome content between strains. However, remarkably, most host-specific differences were restricted to regions of the conserved core genome, which contained allelic variation in genes encoding proteins of known and unknown function. Many of these proteins are predicted to be exported and could play a role in host-pathogen interactions. These data suggest that diversification of the core genome may be more important than acquisition of novel genes for S. aureus host-adaptation. The host-specific determinants identified by the whole-genome approaches adopted in the current study represent excellent targets for studies of the evolution and molecular basis of S. aureus host specificity. Keywords: Strain vs strain
2008-11-19 | GSE10187 | GEO
Project description:The present work describes LC-ESI-MS/MS analyses of tryptic digestion peptides from phages that infect Staphylococcus aureus-causing mastitis, and isolated from dairy products. A total of 1935 non-redundant peptides belonging to 1282 proteins were identified and analyzed. Among them, 80 staphylococcal peptides from phages were confirmed. These peptides belong to proteins such as phage repressors, structural phage proteins, uncharacterized phage proteins and complement inhibitors. Moreover, of the phage origin peptides found, eighteen of them were specific to S. aureus strains. These diagnostic peptides could be useful for the identification and characterization of S. aureus strains that cause mastitis. Furthermore, a study of bacteriophage phylogeny and the relationship among the identified phage peptides and the bacteria they infect was also performed. The results show the specific peptides which are present in closely related phages, and the existing links between bacteriophage phylogeny and the respective Staphylococcus spp. infected.
2021-03-25 | PXD023530 | Pride