ABSTRACT: Gene expression profiles of E15.5 cortical collecting duct and anlage of loop of Henle isolated from developing kidney using LCM. (GUDMAP Series ID: 18)
Project description:NEDD4-2 is an E3 ubiquitin ligase involved in the regulation of several membrane proteins. This project was designed to identify new NEDD4-2-regulated substrates in the mouse cortical collecting duct cell line.
Project description:We profiled SUMO1 and SUMO2 modified proteins in cortical collecting duct (mpkCCD) as a precursor to targeted approaches to assess SUMO function. SUMO levels in cells following heat shock and treatment with deSUMOylation inhibitors were assessed.
Project description:Purpose: Experiments were done in WT mice and Mutant (T551A/S552A at beta-catenin) mice. Cortical collecting ducts (CCD) were manually micro-dissected and utilized for transcriptomic analysis by single-tubule small sample RNA-Seq Methods: We carried out RNA-sequencing in cortical collecting duct (CCD) from WT and MUT mice. Results and conclusion: RNA-Seq in microdissected CCD showed that cell cycle related genes were significanlty changed in the mutant (T551A/S552A) mice.
