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Transcriptomic profile of IEC4.1 cells treated with an ASO to lncRNA XR_001779380 in response to IFN-gamma

ABSTRACT: Transcriptomic profile of IEC4.1 cells treated with an ASO to lncRNA XR_001779380 in response to IFN-gamma

PROVIDER: PRJNA1028577 | ENA |

REPOSITORIES: ENA

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	SRR26398263_1.fastq.gz	Fastqsanger.gz
	SRR26398263_2.fastq.gz	Fastqsanger.gz

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Transcriptomic profile of IEC4.1 cells treated with an ASO to lncRNA XR_001779380 in response to IFN-gamma

Project description:LncRNAs may play an important role in intestinal epithelial cell autonomous defense against microbial infection. Our previous study showed that lncRNA XR_001779380 can interact with Prdm1 to modulate gene transcriuption in intestinal epithelial cells and may regualte IFN-gamma-stimulated gene transcription. Here we designed an antisense oligos-morpholine (ASO4) targeting the sequence of lncRNA XR_001779380 to interfere with its interaction with PRDM1. Murine intestinal epithelial cells (IEC4.1 cells) were treated with ASO4 or a standard control ASO-morpholine (ST) for 24h, followed by exposure to IFN-gamma (1 ng/ml) for 4 hours. Then total RNA was collected for sequencing via DNBSEQ platform to obtain a comprehensive view of the transcriptome.

2025-05-29 | GSE245478 | GEO

Transcriptome Profile of IEC4.1 cells deficient with Prdm1 in response to IFN-gamma

Project description:This study is to investigate the potential role of Prdm1 in murine intestinal epithelial cells (IEC4.1 cells) in response to IFN gamma stimulation. Prdm1 was knocked down in IEC4.1 cells by using a CRISPR/Cas9 approach. Wild type IEC4.1 cells were used as control (Ctrl). Cells were treated with or without mouse IFN gamma (1ng/ml for 4hrs). Then total RNA was collected for sequencing via DNBSEQ platform to obtain a comprehensive view of the transcriptome.

2025-05-30 | GSE245345 | GEO

Transcriptomic profile of IEC4.1 cells treated with an siRNA to lncRNA NR_126553 (Nostril) in response to IFN-gamma protein (IFNγ)

Project description:This study is to investigate the potential impact of an lncRNA NR_126553 (Nostril) in murine intestinal epithelial cells (IEC4.1 cells) in response to IFN-gamma protein (IFNγ) stimulation. NR_126553 (Nostril) was knocked down by using a pool of gene specific siRNA (SiNostril). Cells treated with scramble non-specific siRNA were used as control (siNegative control). After siRNA transfection 24h, cells were treated with or without mouse IFNγ (1ng/ml for 4hrs). Then total RNA was collected for sequencing via DNBSEQ platform to obtain a comprehensive view of the transcriptome.

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Transcriptome Profile of IEC4.1 cells deficient with Prdm1 in response to IFN-gamma

Project description:Transcriptome Profile of IEC4.1 cells deficient with Prdm1 in response to IFN-gamma

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Transcriptomic profile of IEC4.1 cells treated with an siRNA to lncRNA NR_126553 (Nostril) in response to IFN-gamma protein (IFNγ)

Project description:Transcriptomic profile of IEC4.1 cells treated with an siRNA to lncRNA NR_126553 (Nostril) in response to IFN-gamma protein (IFNγ)

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human ESC-MSCs: IFN-γ-nontreated, IFN-γ-treated for 1 day, IFN-γ-treated for 3 days

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human ESC-MSCs: IFN-γ-nontreated, IFN-γ-treated for 1 day, IFN-γ-treated for 3 days

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IFN-gamma priming

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Transcription profiling of IFN-gamma transcriptional responses in control and IFN-gamma primed primary human macrophages

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2007-09-01 | E-GEOD-1925 | biostudies-arrayexpress

The effects of terbutaline or GW9508 on TNF-alpha and IFN gamma (TNF-alpha + IFN gamma) stimulation by HaCaT

Project description:G-protein coupled receptors (GPCRs) have diverse roles in physiological processes, including immunity. Gs-coupled GPCRs increase while Gi-coupled ones decrease intracellular cAMP. Previous studies suggest that, in epithelial cells, Gs-coupled GPCRs enhance whereas Gi-coupled GPCRs suppress pro-inflammatory immune responses. In order to examine the issue, we chose beta2 adrenergic receptor and GPR40 as representatives of Gs- and Gi- coupled GPCRs, respectively, and examined their effects on TNF-alpha and IFN-gamma-(TNF-alpha + IFN-gamma) induced gene expression by HaCaT. We used microarrays to detail the global changes of gene expression induced by a beta2 adrenergic receptor agonist terbutaline or GPR40 agonist GW9508 pre-treatment in TNF-alpha + IFN-gamma - stimulated HaCaT cells. HaCaT cells were pre-treated with terbutaline or GW9508, TNF-alpha + IFN-gamma were then added, and cultured for another 24 h. Cells were then used for RNA extraction and hybridization on Affymetrix microarrays. We sought to clarify changes in gene expression after 1) TNF-alpha + IFN-gamma, 2) TNF-alpha + IFN-gamma + terbutaline, and 3) TNF-alpha + IFN-gamma + GW9508 treatment. To this end, we set 4 groups of samples; 1) unstimulated group, 2) TNF-alpha + IFN-gamma-stimulated group, 3) TNF-alpha + IFN-gamma + terbutaline-stimulated group, and 4) TNF-alpha + IFN-gamma + GW9508-stimulated group. In each group, HaCaT cells were stimulated in triplicate wells (n=3).

2010-12-01 | E-GEOD-20297 | biostudies-arrayexpress

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