Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Synthetic human type 1 collagen peptides C1a1T201

PROVIDER: PRJNA1035289 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR26661891.fastq.gz	Fastqsanger.gz

Items per page:

1 - 1 of 1

Similar Datasets

Next generation seuquencing of 3D breast cancer cell culture in synthetic hydrogels presenting either a collagen mimic or laminin mimic

Project description:MDA-MB-231 (ER-) and T47D (ER+) breast cancer cells were encapsulated in synthetic hydrogels, formed with a multi-arm bioinert polymer poly(ethylene glycol), cell-degradable peptide crosslinks, and receptor-binding pendant peptides, and cultured for 3 days to assess initial cell responses to different matrix compositions. The synthetic matrices had a Young's modulus (E) ~ 0.6 kPa, in the range of bone marrow and healthy mammary tissue, and biochemcial cues to mimic binding sites found in collagen (GFOGER peptide) or laminin (IKVAV peptide). After 3 days in culture, mRNA was collected and RNA-seq performed to compare cell responses between the collagen and laminin mimetic cultures.

2020-02-20 | GSE121179 | GEO

Project description:RNA sequencing of human dermal fibroblast treated with collagen peptides

| PRJNA1143071 | ENA

Next generation seuquencing of 3D breast cancer cell culture in synthetic hydrogels presenting either a collagen mimic or laminin mimic with low and high mechanical properties

Project description:MDA-MB-231 (ER-) and T47D (ER+) breast cancer cells were encapsualted in synthetic hydrogels, formed with a multi-arm bioinert polymer poly(ethylene glycol), cell-degradable peptide crosslinks, and receptor-binding pendant peptides, and cultured for 3 days and up to 40 days to assess initial cell responses to different matrix compositions. The synthetic matrices had a Young's modulus (E) ~ 0.6 kPa (low wt%) and # ~ 5 kPa (high wt%), in the range of bone marrow or healthy mammary tissue and lung tissue, respectively, and Biochemcial cues to mimic binding sites found in collagen (GFOGER peptide) or laminin (IKVAV peptide). After 3 days and 40 days in culture, mRNA was collected and RNA-seq performed to compare cell responses between the collagen and laminin mimetic cultures overtime in both low wt% and high wt% matrices.

2022-03-01 | GSE145696 | GEO

Transcription profiling of human CD4+ T cells induced by HIV-1 gp120/V3 loop peptides, time series

Project description:Comparative changes of gene expression in human primary CD4+ T cells induced by HIV-1 gp120/V3 loop synthetic peptides, during antigen presentation

2008-09-01 | E-MEXP-1586 | biostudies-arrayexpress

Global gene expression profiling of tissue engineered neofascia of hernia patients with good and bad healing characterisitics.

Project description:The prevalence of hernia after laparatomy has been estimated at 2-15%. Apart from male gender, smoking and obesity (BMI>25), increased age (>45 years) were identified as potential risk factors. To support the wound healing process, synthetic mesh is now routinely used in laparatomy. A patient's risk for hernia formation and relapse can be estimated by his/her collagen type I / collagen type III ratio using sirius red stains.

2020-06-01 | GSE66818 | GEO

Effect of hIAPP aggregation on gene expression by mouse bone marrow-derived macrophages after 12 hours

2010-08-11 | GSE23534 | GEO

Water-soluble tomato extract Fruitflow alters the phosphoproteomic profile of collagen-stimulated platelets

Project description:The present study used proteomics to quantitatively analyze the effect of FF on the inhibition of collagen-stimulated platelets and validated the involvement of several signaling molecules.Fruitflow significantly inhibited human platelet aggregation and P-selectin expression that were induced by collagen. Proteomics analysis revealed that 60 proteins were upregulated in FF-treated collagen-stimulated platelets, and 10 proteins were downregulated in collagen-stimulated platelets alone. Additionally, 66 phosphorylated peptides were upregulated in FF-treated collagen-stimulated platelets, whereas 37 phosphorylated peptides were downregulated in collagen-stimulated platelets alone.

2021-08-10 | PXD027834 |

FIA plasma induces arthritis in naïve mice

Project description:Plasma collected from mice with FIA were pooled, and 0.3 ml was injected intravenously into 6-week-old naïve SJL mice on days 0 and 2. Synovial antigen array profiling of plasma from the arthritic recipient mice demonstrated autoreactive B-cell responses against peptides representing native fibrinogen and citrullinated fibrinogen, and further epitope spreading resulting in additional targeting of fibronectin, collagen type V, cartilage gp39, and clusterin.

2009-09-12 | GSE18024 | GEO

Effect of hIAPP aggregation on gene expression by mouse bone marrow-derived macrophages after 12 hours

Project description:The purpose of this experiment was to determine changes in gene expression by bone marrow-derived macrophages (BMDMs) treated with synthetic human vs. rodent islet amyloid polypeptide (IAPP). Synthetic human IAPP at 15 uM aggregates to form fibrils in vitro, whereas rodent IAPP is non-amyloidogenic. We hypothesized that interaction of macrophages with human IAPP aggregates can activate pro-inflammatory signalling pathways in macrophages, as described for other amyloidogenic peptides. This array includes eight samples from one experiment, with two groups of four replicates each. Each replicate represents BMDMs from one well of a 24-well plate. The control group was treated with 15 uM rat IAPP for 12 hours prior to total RNA extraction; the experimental group was treated with 15 uM human IAPP.

2010-08-11 | E-GEOD-23534 | biostudies-arrayexpress

Identification of collagen marker peptides for species identification of glue

Project description:To establish an LC-MS method for identification of the animal source of glue, we performed MS/MS identification of type I collagen-derived marker peptides using authentic collagen, glue, and leather samples after trypsin digestion.

2018-07-18 | PXD009059 | JPOST Repository

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data