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Glutathione triggers leaf-to-leaf, calcium-based plant defense signaling

ABSTRACT: Glutathione triggers leaf-to-leaf, calcium-based plant defense signaling

PROVIDER: PRJNA1049785 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27130014_1.fastq.gz	Fastqsanger.gz
	SRR27130014_2.fastq.gz	Fastqsanger.gz
	SRR27130015_1.fastq.gz	Fastqsanger.gz
	SRR27130015_2.fastq.gz	Fastqsanger.gz
	SRR27130016_1.fastq.gz	Fastqsanger.gz

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Glutathione triggers leaf-to-leaf, calcium-based plant defense signaling

Project description:To get deeper insights into the GSH-triggered systemic defense signaling events in plants, we performed transcriptome profiling on leaf 6 collected after one hour application of GSH to leaf 1 of WT plants. To further investigate the relations between GSH and Glu in plant wound response, we generated transcriptome data sets from leaf 6 one hour after wounding leaf 1 or application of Glu to leaf 1 of WT plants.

2025-01-06 | GSE249592 | GEO

Glutathione triggers leaf-to-leaf, calcium-based plant defense signaling.

Project description:Animals rely on nervous systems to cope with environmental variability, whereas plants are characterized by lack of nervous system but still have evolved systemic communication systems through signaling molecules that trigger long-distance defense signaling events when encountered with environmental challenges. Here, our genetic screening of the previously constructed hairpin RNA-based Arabidopsis library identifies a glutathione (GSH)-deficient mutant that has high accumulation of glutamate (Glu), a previously defined wound signal essential for activating plant defense, but disharmoniously exhibits attenuation of defense signaling events. We further uncover GSH as a critical signaling molecule that relies on GLUTAMATE RECEPTOR-LIKE 3.3 (GLR3.3) to trigger long-distance calcium-based defense signaling events in plants. Our findings offer new insights into highly sophisticated systemic defense systems evolved by plants to defend against herbivory and pathogen invasion.

| S-EPMC11850895 | biostudies-literature

Glutathione-induced elicitation of defense responses in Lotus japonicus.

Project description:To identify transcription factors involved in the production of defense-related phytochemicals through co-expression studies. The changes in gene expression following GSH elicitation of leaves was followed over a time course. Four biological replicates of the unelicited samples and three biological replicates were used for each time point.

2011-09-15 | E-GEOD-31240 | biostudies-arrayexpress

Glutathione-induced elicitation of defense responses in Lotus japonicus.

Project description:To identify transcription factors involved in the production of defense-related phytochemicals through co-expression studies.

2011-09-16 | GSE31240 | GEO

Inositol triphosphate-triggered calcium release blocks lipid exchange at endoplasmic reticulum-Golgi contact sites

Project description:Vesicular traffic and membrane contact sites between organelles enable the exchange of proteins, lipids, and metabolites. Recruitment of membrane tethers to contact sites between the endoplasmic reticulum (ER) and the plasma membrane is often triggered by calcium. In contrast, we reveal here a function for calcium in the repression of cholesterol export at membrane contact sites between the ER and the Golgi complex. We show that calcium efflux from ER stores induced by inositol-triphosphate [IP3] accumulation upon loss of the inositol 5-phosphatase INPP5A or sustained receptor signaling triggers the depletion of cholesterol and associated complex glycosphingolipids from the cell surface, resulting in a blockade of clathrin-independent endocytosis (CIE) of bacterial Shiga toxin. This phenotype is caused by the calcium-induced dissociation of oxysterol binding protein (OSBP) from the Golgi complex and from VAP-containing membrane contact sites. Our findings reveal a crucial function for INPP5A-mediated IP3 hydrolysis in the control of lipid exchange at membrane contact sites.

2021-05-06 | MTBLS2444 | MetaboLights

Priming defense genes and metabolites in hybrid poplar by the green leaf volatile cis-3-hexenyl acetate

Project description:• Herbivore-induced plant volatiles (HIPVs), in addition to attracting natural enemies of herbivores, can serve a signaling function within plants by acting as wound signals that induce or prime defenses. However, particularly in woody plants, which compounds within HIPV blends are capable of acting as signaling molecules are largely unknown. • Leaves of hybrid poplar (Populus deltoides x nigra) saplings were exposed in vivo to naturally wound-emitted concentrations of the green leaf volatile (GLV) cis-3-hexenyl acetate (z3HAC) and then subsequently fed upon by gypsy moth larvae (Lymantria dispar L.). Volatiles were collected throughout the experiments, and leaf tissue was collected to measure phytohormone levels and expression of defense-related genes. • Relative to controls, z3HAC-exposed leaves had higher levels of jasmonic acid and linolenic acid following gypsy moth feeding. Further, z3HAC primed transcripts of phytohormone signaling (lipoxygenase 1) and direct defense (a Kunitz proteinase inhibitor) genes. These qRT-PCR results were supported by microarray analysis using the AspenDB 7K EST microarray containing ~5400 unique gene models. Moreover, z3HAC also primed the release of herbivore-induced terpene volatiles. • The widespread priming response suggests an adaptive benefit to detecting z3HAC as a wound signal. Thus, woody plants can detect and use z3HAC as a signaling cue to prime defenses before actually experiencing damage. GLVs may therefore have important ecological functions in arboreal ecosystems.

2008-07-24 | GSE11955 | GEO

Competition for energy between immunity and homeothermy triggers trade-offs and regulates host defense strategies

Project description:Competition for energy between immunity and homeothermy triggers trade-offs and regulates host defense strategies

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RNAseq transcriptome analysis of Streptococcus pyogenes HKU16 and an isogenic gshT mutant. Manuscript title: Streptococcus pyogenes hijacks host glutathione for growth and innate immune evasion

Project description:The nasopharynx and the skin are the major oxygen-rich anatomical sites for colonization by the human pathogen Streptococcus pyogenes (group A Streptococcus, GAS). To establish infection, GAS must survive oxidative stress generated during aerobic metabolism and the release of reactive oxygen species (ROS) by host innate immune cells. Glutathione is the major host antioxidant molecule while GAS is glutathione-auxotrophic. Here we report the molecular characterization of the ABC transporter substrate binding protein GshT in the GAS glutathione salvage pathway. We demonstrate that glutathione uptake is critical for aerobic growth of GAS and that impaired import of glutathione induces oxidative stress that triggers enhanced production of the reducing equivalent NADPH. Our results highlight the interrelationship between glutathione assimilation, carbohydrate metabolism, virulence factor production and innate immune evasion. Together, these findings suggest an adaptive strategy employed by extracellular bacterial pathogens to exploit host glutathione stores for their own benefit.

2022-04-04 | GSE198061 | GEO

Expression data of glutathione-fed Arabidopsis thaliana

Project description:To comprehensively investigate the effects of glutathione on the gene expression, the microarray analysis was performed in the glutathione-fed wild-type Arabidopsis thaliana. Wild-type Arabidopsis (ecotype Columbia-0) were fed with 1 mM oxidized glutathione (GSSG) and 2 mM reduced glutathione (GSH) for comparison at equal nitrogen equivalents. To examine the effects of glutathione other than nitrogen at equal nitrogen equivalents, plants were fed with 3 mM NH4NO3. Plants grown by water were used as a control.

2023-08-01 | GSE228403 | GEO

Interactions between H2O2 and glutathione in gene expression

Project description:rs08-03_glutathion - glutathion - How does glutathione content or reduction state affect H2O2-induced changes in the transcriptome? - Three single Arabidopsis mutants were used: cat2, knockout for catalase2 and so enriched in H2O2; cad2, defective in glutathione content; cytGR, knockout for cytosolic glutathione reductase. Cat2 was crossed with cad2 and cyt GR, and col0, 3 single mutants, and 2 double mutants were sampled in controlled growth conditions either in 8h or 16h photoperiod. Keywords: gene knock out 20 dye-swap pairs - CATMA arrays: 40 arrays

2010-07-22 | E-GEOD-21424 | biostudies-arrayexpress

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