Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1051664 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Isolates from the Japanese Veterinary Antimicrobial Resistance Monitoring System(JVARM : PRJDB8154)
| PRJDB20742 | ENA
Project description:National Antimicrobial Resistance Monitoring System (NARMS)
| PRJNA290865 | ENA
Project description:National Antimicrobial Resistance Monitoring System (NARMS) Water Metagenome
| PRJNA794347 | ENA
Project description:Mesenchymal Stromal Cells (MSCs) are widely studied in regenerative medicine because of their unique biological properties. The MSC-derived secretome is a cell-free product containing soluble factors and extracellular vesicles that mediate many of the biological properties of MSCs, including immunomodulatory and antimicrobial activities. This study aimed to evaluate the antimicrobial activity of a freeze-dried secretome derived from canine adipose tissue MSCs (canine lyosecretome, c-Lyo) against pathogens relevant to canine infections. The antimicrobial activity of c-Lyo was assessed using minimal inhibitory concentration (MIC) assays against six bacterial species (Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus pseudintermedius and Pseudomonas aeruginosa) and one yeast (Malassezia pachydermatis). Potential interactions between c-Lyo and commonly used veterinary antimicrobials (gentamicin, amoxicillin, enrofloxacin, and ketoconazole) were evaluated using checkerboard assays (CkA). Proteomic characterization of the c-Lyo and transcriptomic analysis of canine MSCs were also conducted to explore molecular mechanisms underlying antimicrobial activity. The results demonstrated that c-Lyo exerts antimicrobial activity against all tested microorganisms, with variable sensitivity among species. CkA exhibited predominantly additive interactions with conventional antimicrobials and occasional synergistic effects, with no evidence of antagonism. Proteomic and transcriptomic analyses supported the presence of antimicrobial peptides and immune-related pathways that potentially contribute to both direct antimicrobial activity and the modulation of host defense mechanisms. These findings indicate that c-Lyo may represent a promising cell-free biologic approach for antimicrobial strategies in veterinary medicine.
2026-04-09 | GSE327506 | GEO
Project description:We previously showed that doxycycline and carprofen , a veterinary non-steroidal anti-inflammatory drug, have synergistic antimicrobial activity against methicillin-resistant Staphylococus pseudintermedius (MRSP) carrying the tetracycline resistance determinant TetK. To elucidate the molecular mechanism of this synergy, we investigated the effects of the two drugs, individually and in combination, using a comprehensive approach including two-dimensional differential in-gel electrophoresis (2D DIGE).
2020-05-26 | PXD013725 | Pride
Project description:“Viable but non-culturable” (VBNC) states pose challenges for environmental and clinical microbiology, but their biological mechanisms remain obscure. Mycobacterium tuberculosis (Mtb), the leading cause of death from infection until COVID-19, affords a striking example. Mtb can enter into a “differentially detectable” (DD) state associated with phenotypic antimicrobial resistance in which Mtb cells are viable but undetectable as colony-forming units. We found that Mtb cells enter the DD state when they undergo sublethal oxidative stress that damages their DNA, proteins, and lipids, and in addition, their replication is delayed, allowing repair. Mycobacterium bovis and BCG fail to enter the DD state under similar conditions. These findings have implications for TB latency, detection, relapse, treatment monitoring, and development of regimens that overcome phenotypic antimicrobial resistance.
2021-11-24 | GSE180792 | GEO