Project description:We report on the implications of genetic KO versus MO-mediated KD of the mesoderm-specifying Brachyury paralogues in the Western clawed frog Xenopus tropicalis. While both KO and KD embryos fail to activate the same core gene regulatory network, resulting in virtually identical morphological defects, embryos injected with control or target MOs also show a systemic GC content dependent immune response and many off-target splicing defects.
Project description:Proteomic analysis of responses to cold exposure in the livers of two frog species from distinct habitats: the African clawed frog (Xenopus laevis) and the Northeastern Asian brown frog (Rana dybowskii), and proteomics analysis of mouse liver during cold storage.
