Project description:Identifying the differentially expressed proteins of Riemerella anatipestifer CH-1 and CH-1_zntR under TSB condition through proteomic analysis
Project description:Identifying the differentially expressed genes of Riemerella anatipestifer CH-1 and CH-1_zntR under normal condition through RNA sequencing.
2025-01-18 | GSE286539 | GEO
Project description:Transcriptome sequencing of Riemerella anatipestifer CH-1 and CH-1_mntR under TSB condition
| PRJNA909940 | ENA
Project description:Transcriptome sequencing of Riemerella anatipestifer CH-1 and CH-1_zntR under TSB condition
Project description:We performed comparative transcriptomic analysis of the outer membrane vesicles (OMVs) released from B. burgdorferi. We identified a total of ~1200 unique transcripts with at least one mapped read from the bacterial cell and its OMVs.
Project description:The alveolar epithelial cell injury is essential for the initaitation and maintenance of inflammatory of lung in ARDS. Outer membrane vesicles (OMVs) are nanoparticles produced by the extrusion of the outer membrane of gram-negative bacteria deliver a range of bacterial molecules to host cells. Recent findings describe OMVs play vital roles in bacterial colonization, delivery of virulence factors, and disease pathogenesis. We here report OMVs as a key regulator of gene expression in primary alveolar epithelial cell.
Project description:Infectious serositis of ducks caused by Riemerella anatipestifer infection has brought great losses to poultry industry all over the world. Adhesion and invasion were important pathway of the infection of Riemerella anatipestifer. Gram-negative pathogens’ adhesion and invasion of host cells by means of outer membrane proteins was a common phenomenon. Here, by using LC-MS/MS, we determined the adhesins belonging to the outer membrane protein of Riemerella anatipestifer RA-YM strain.
2024-10-27 | PXD057236 |
Project description:Transcriptome sequencing of Riemerella anatipestifer CH-1 under iron-repleted condition and iron-depleted condition
Project description:Riemerella anatipestifer can cause infectious serositis in ducklings with high mortality. As a bacterial adhesin receptor, membrane cofactor CD46 plays a role in the process of bacterial adhesion to the host. Outer membrane protein (OMP) is necessary for Gram-negative bacteria to adhere, colonize and cause diseases. Therefore, we used duck CD46 protein (dCD46) as bait protein, and screened out the OMPs of Riemerella anatipestifer which can interact with dCD46 by pull-down test, and searched for the adhesion protein of Riemerella anatipestifer, which laid a foundation for the prevention of Riemerella anatipestifer.
Project description:Carbapenem-resistant Acinetobacter baumannii (CRAB) is a critical nosocomial pathogen with limited treatment options. Although antibiotic resistance in CRAB is well-characterized, its interactions with host immunity and the contribution of outer membrane vesicles (OMVs) to pathogenesis remain poorly understood. We examined a clinical CRAB isolate and compared it with the reference strain A19606. Antimicrobial susceptibility testing revealed complete resistance of CRAB to commonly used antibiotics in clinical practice, while A19606 remained susceptible to most agents. In murine intranasal infection models and bone marrow-derived macrophages, CRAB induced significantly stronger activation of inflammatory signaling pathways and elevated levels of pro-inflammatory cytokines relative to A19606. Transcriptomic analysis of infected lung tissue identified differentially expressed genes, enriched for inflammatory response pathways. proteomics showed upregulated proteins in CRAB related to secretion systems. OMVs characterization revealed that CRAB-derived OMVs highly enriched in proteins associated with periplasmic and outer membrane spaces, and more potent in triggering macrophage inflammatory signaling. CRAB displays expansive antibiotic resistance and enhanced pro-inflammatory potential mediated in part by unique OMVs properties. Targeting OMVs formation or host immune modulation may represent effective strategies for combating CRAB infections.