ABSTRACT: Characterization of the complete chloroplast genome of purple flowering stalk (Brassica campestris var. purpuraria) and comparisons with related
Project description:Heterologous expression of a water soluble chlorophyll protein from Brassica oleracea var. gemmifera in the phototrophic purple bacterium Rhodobacter sphaeroides was verified by bottom-up proteomic analysis.
Project description:Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is one of the most devastating diseases of cruciferous crops worldwide. The pathogen infects and multiplies in plant vascular tissues and, as the disease progresses, the veins of infected tissues turn black and characteristic V-shaped lesions appear along the margins of leaves.The aim of this work is to identify differentially expressed genes from Brassica oleracea during early infection by Xcc, in an attempt to identify proteins related to resistance. Cabbge seedlings were inoculated with Xanthomonas campestris pv campestris (Xcc) suspension and cabbage gene expression at 6h., 24h. And 48h. After inoculation was assessed with help of Brassica 95k EST microarray chip.
Project description:Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is one of the most devastating diseases of cruciferous crops worldwide. The pathogen infects and multiplies in plant vascular tissues and, as the disease progresses, the veins of infected tissues turn black and characteristic V-shaped lesions appear along the margins of leaves.The aim of this work is to identify differentially expressed genes from Brassica oleracea during early infection by Xcc, in an attempt to identify proteins related to resistance.
Project description:Brassica nigra plants, a Brassicaceae close to Arabidopsis thaliana, was used for combined stresses experiments. In this study, we performed a whole-genome microarray analysis on five-week-old plants and compared untreated plants and plants treated different single or dual stresses: the larvae Pieris brassicae, egg extract of Pieris brassicae, the bacterial Xanthomonas campestris pv. raphani, the aphid Brevicoryne brassicae or by combined stresses eggs of P. brassicae / P. brassicae, X. campestris / P. brassicae, B. brassicae / P. brassicae.
Project description:Understanding plant’s response mechanisms against pathogenesis is fundamental for the development of resistant crop varieties and more productive agriculture. In this regard, ‘omic’ approaches are heralded as valuable technologies. In this work, combining iTRAQ technology with mass spectrometry, the proteomes from leaves of Brassica oleracea plants infected with Xanthomonas campestris pv. campestris (Xcc) and control plants at two different post-infection times were compared.
Project description:Understanding plant’s response mechanisms against pathogenesis is fundamental for the development of resistant crop varieties and more productive agriculture. In this regard, ‘omic’ approaches are heralded as valuable technologies. In this work, combining iTRAQ technology with mass spectrometry, the proteomes from leaves of Brassica oleracea plants infected with Xanthomonas campestris pv. campestris (Xcc) and control plants at two different post-infection times were compared.