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DICER is downregulated in mouse adipose tissue during high-fat diet feeding

ABSTRACT: DICER is downregulated in mouse adipose tissue during high-fat diet feeding

PROVIDER: PRJNA1070687 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27766134.fastq.gz	Fastqsanger.gz
	SRR27766135.fastq.gz	Fastqsanger.gz
	SRR27766136.fastq.gz	Fastqsanger.gz
	SRR27766137.fastq.gz	Fastqsanger.gz
	SRR27766138.fastq.gz	Fastqsanger.gz

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DICER is downregulated in mouse adipose tissue during high-fat diet feeding

Project description:The adipose tissue plays an important role in controlling whole-body energy balance, and proper regulation of adipose tissue function is essential for metabolic health. In response to energy surplus, the adipose tissue needs to expand, which may lead to local areas of hypoxia within the tissue. This is thought to promote whole-body insulin resistance. Here we report that DICER, a key enzyme in the maturation of miRNAs and a potential marker of adipocyte health, is profoundly downregulated in mouse adipose tissue within the first week of high-fat diet (HFD) feeding, and this effect is sustained in response to long-term HFD feeding. The downregulation of DICER protein occurs in both mature adipocytes and in the stromal vascular cells. Mechanistically, we provide evidence that hypoxia and hypoxia-inducible factor 1-α (HIF1α) facilitate ubiquitination of DICER to target it for autophagy-mediated degradation, and we show that DICER and HIF1α interact in adipose tissue after HFD feeding, which may signal for DICER degradation. Finally, despite reductions in DICER protein, we were not able to detect any differences in global miRNA levels in subcutaneous adipose tissue of mice after one or three weeks of HFD-feeding. In conclusion, the nutritional challenge of HFD feeding in mice leads to a large reduction in adipose tissue DICER protein, which is induced by hypoxia during tissue expansion and mediated through an interaction with HIF1α.

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Effect of high fat diet feeding on adipose tissue macrophages

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Genome-wide analysis of gene expression in adipose tissue of female mice after continuous high fat diet feeding

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2013-05-10 | E-GEOD-37238 | biostudies-arrayexpress

Genome-wide analysis of gene expression in adipose tissue of female mice after continuous high fat diet feeding

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Fat transcriptome patterns associated with heterogeneous metabolic, hormonal and behavioral adaptation to high fat diet feeding in mice

Project description:The effect of high fat diet feeding on adipose tissue gene transcription regulation was investigated in C57Bl/6J mice using Affymetrix gene expression arrays. Expression profiling was determined in 5 months old male mice showing heterogeneous metabolic, hormonal and behavioral adaptation to high fat diet (40% fat) feeding for 15 weeks. Control mice were fed a standard carbohydrate chow. Six animals per group were used.

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Integrated proteome and phosphoproteome analysis of interscapular brown adipose and subcutaneous white adipose tissues upon high fat diet feeding in mouse

Project description:Thermogenesis is a promising approach to limit weight gain in response to excess nutrition. In contrast to cold-induced thermogenesis, the molecular and cellular mechanisms of diet-induced thermogenesis (DIT) have not been fully characterized. Here, we explored the response of brown adipose tissue (BAT) and beige adipose tissue to high fat diet (HFD) using proteome and phosphoproteome analysis. We observed that after HFD, DIT was only activated in BAT. Furthermore, fatty acid oxidation, tricarboxylic acid cycle, and oxidative phosphorylation were also activated in BAT. Nevertheless, most metabolic pathways downregulated in beige adipose tissue. Strikingly, we found that these metabolic changes accompanied with different variation of mitochondria between BAT and beige adipose tissue as well. HFD treatment impaired mitochondrial functions and mitochondrial protein synthesis in beige adipose tissue while it stimulated mitochondrial autophagy in BAT. Together, in BAT, HFD caused increased mitochondrial activity,

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Effect of high fat diet feeding on adipose tissue macrophages

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Transcriptomic profiling of murine adipose endothelial cells from high-fat diet fed mice

Project description:Female mice display greater adipose angiogenesis and maintain healthier adipose tissue than do males upon sustained high-fat diet feeding. We utilized transcriptome analysis of endothelial cells (EC) from the white adipose tissue of male and female mice that were fed a high-fat for 7 weeks. We found that adipose EC exhibited pronouncedly sex-distinct transcriptomes. Genes upregulated in female adipose EC were associated with proliferation, oxidative phosphorylation, and chromatin remodeling contrasting the dominant enrichment for genes related to inflammation and a senescence-associated secretory of male EC. Our study provides novel insights into molecular programs that distinguish male and female EC responses to pathophysiological conditions.

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