Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1077338 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR27991700_1.fastq.gz | Fastqsanger.gz | |||
| SRR27991700_2.fastq.gz | Fastqsanger.gz | |||
| SRR27991800_1.fastq.gz | Fastqsanger.gz | |||
| SRR27991800_2.fastq.gz | Fastqsanger.gz | |||
| SRR27991701_1.fastq.gz | Fastqsanger.gz |
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Project description:Preeclampsia is a devastating hypertensive disorder of pregnancy that afflicts between 5-10% of pregnancies in the US, with a higher prevalence in the southern regions of the United States. Black pregnant women are disproportionally at higher risk for preeclampsia onset and severity of disease than other racial groups, including White women. Although this has been recognized in the maternal fetal medicine literature for many years, the underlying mechanism(s) for this racial disparity are unclear. One confounding issue with many studies of racial discrepancies in pregnant women is differences in comorbidities between racial groups. In our current study we were able to obtain a cohort of patients of self-identifying Black or White race that were overall matched for BMI, blood pressure, gestational age and parity and we hypothesized that genetic and placental markers of preeclampsia would be highest in our preeclampsia Black patients compared to healthy patients or White preeclampsia patients. We collected placenta tissue from this cohort of deliveries in Augusta, GA from both healthy and preeclamptic Black and White women (n=13-15). We measured expressions of several preeclampsia-implicated genes, as well as performed a placental morphological analysis and RNA sequencing of a subset of samples. Contrary to our hypothesis, our results from ddPCR analysis of genes associated with preeclampsia risk demonstrated that leptin, preproendothelin-1 (PPET-1) endothelial converting enzyme-1 (ECE-1) and soluble FMS-like tyrosine kinase-1 (sFlt-1) were higher in White preeclamptic women than in Black preeclamptic women. Our RNA sequencing analysis revealed that 136 genes significantly differed between White and Black normal pregnant patients, 288 genes differed between Black healthy and Black preeclampsia patients, while a striking 2394 genes significantly differed between White healthy and White preeclampsia patients and 2346 genes differed between White and Black preeclampsia patients. Placental histology analysis revealed that Black preeclampsia patients demonstrated a lower scoring of morphological pathologies associated with preeclampsia compared to White preeclampsia patients. Collectively, these data indicate that genetic predisposition to preeclampsia in White patients in the placenta differs greatly to that of Black patients, and that placental changes may be more subtle in Black patients that are at high risk for preeclampsia.
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Project description:Preeclampsia is one of the most common pregnancy disorders, and characterized by insufficient trophoblast invasion and placental inflammation. RNA sequencing was performed using placental tissues collected from PE patients and control healthy pregnant women. The results showed that OTU deubiquitinase, ubiquitin aldehyde binding 2 (OTUB2) was downregulated in placenta from PE patients.
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Project description:Preeclampsia is a disease of pregnant women, which is characterized by hypertension, proteinuria and chronic inflammation. There is a growing body of evidence that cause of preeclampsia lies within immunological aspect of pregnancy. This study aimed to analyze the role of CD74 in preeclampsia with a focus on its influence on communication between placental macrophages (Hofbauer cells) and trophoblasts. We have found CD74 to be highly dysregulated in preeclamptic placenta by real-time RT-PCR and Western blot methods. We identified Hofbauer cells to express the highest levels of CD74 in placenta by immunofluorescence and flow cytometry and that CD74 in preeclamptic Hofbauer cells is lower than in controls. We have performed a transcriptome analysis on human blood monocyte-derived macrophages that were non- or IL-4-activated and treated with small interfering RNA against CD74 (siRNA CD74) or non-targeting siRNA (siRNA non-targeting) as control.
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Project description:Preeclampsia (PE) is a multifactorial disease with a complex pathophysiology and no specific treatment currently available. The release of harmful factors into maternal circulation and endothelial dysfunction play a key role in the development of preeclampsia. This study aims to identify altered proteins in endothelial cells exposed to the plasma of pregnant women with early and late onset PE comparing them with cells exposed to plasma from healthy pregnant women.
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Project description:Preeclampsia is a common complication of pregnancy that affects 4-5% of pregnant women around the world. At present, there is a lack of early identification of high-risk patients of preeclampsia in clinical practice, which restricts the development of disease prevention and treatment. Previous studies have indicated that plasma exosomal miRNAs in pregnant women could serve as biomarkers of preeclampsia, but few is focused on exosomal miRNAs from preeclampsia pregnancy with severe features(sPE). Therefore, we detected and compared the plasma exosomal miRNA profiles between normal pregancy and sPE to explore potential biomarkers and pathogenic mechanisms of sPE.
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