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GEO accession GSE256054 is currently private and is scheduled to be released on Jul 15, 2025.

ABSTRACT: GEO accession GSE256054 is currently private and is scheduled to be released on Jul 15, 2025.

PROVIDER: PRJNA1077948 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28006373_1.fastq.gz	Fastqsanger.gz
	SRR28006373_2.fastq.gz	Fastqsanger.gz
	SRR28006374_1.fastq.gz	Fastqsanger.gz
	SRR28006374_2.fastq.gz	Fastqsanger.gz
	SRR28006375_1.fastq.gz	Fastqsanger.gz

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IGF2BP3 Inhibits Translation in Round Spermatozoa

Project description:To reveal the effects of IGF2BP3 on mouse spermatids at the post-transcriptional and translational levels, we performed RNA-seq and ribosome nascent-chain complex sequencing (RNC-seq) of spermatids from Igf2bp3+/- and Igf2bp3-/- mice after isolation. RNA-seq and RNC-seq sequencing showed that IGF2BP3 mainly plays a regulatory role in translational repression in spermatids.

2025-11-30 | GSE256054 | GEO

De novo protein synthesis occurs through cytoplasmic translation machinery in mammalian spermatozoa

Project description:For decades, it was widely accepted that mammalian spermatozoa were devoid of translational activity due to the functional and morphological changes that occurred during spermatogenesis. However, it was proved that capacitated spermatozoa possess translation activity, which appears to be ensured by mitochondrial ribosomes. Since mitochondrial translation has several particularities, the hypothesis that sperm translation occurs through mitochondrial ribosomes raises some questions. The main objectives of this work were to study the impact of translation inhibition in the bovine spermatozoa proteome and to identify the de novo synthesized proteins by capacitated bovine spermatozoa. To identify which proteins were de novo translated, capacitated bovine spermatozoa were treated with puromycin and puromycin-peptides were immunoprecipitated. To study the impact of translation inhibitors in capacitated sperm proteome, bovine spermatozoa were capacitated in the presence or absence of translation inhibitors - Cycloheximide (CHX, cytoplasmic translation inhibitor) and D-Chloramphenicol (D-CP, mitochondrial translation inhibitor). After capacitation, sperm lysates were analysed by mass spectrometry. We identify nine newly synthesised cytoplasmic proteins by capacitated bovine spermatozoa, which are related to sperm-specific processes. Both translation inhibitors impact differently the sperm proteome leading to the deregulation of cytoplasmic protein related to sperm physiology and function. These results support the existence of cytoplasmic translation in bovine spermatozoa during capacitation.

2025-06-13 | PXD050868 | Pride

The IGF2BP3-COPS7B axis facilitates mRNA translation to drive colorectal cancer progression

Project description:Many studies have provided valuable information about genomic and transcriptomic changes that occur in colorectal cancer (CRC). However, protein abundance cannot be reliably predicted by DNA alteration or mRNA expression, which can be partially attributed to post-transcriptional and/or translational regulation of gene expression. In this study, we identified increased translational efficiency (TE) as a hallmark of CRC by evaluating the transcriptomic and proteomic features of CRC patients, along with comparative transcriptomic and ribosome-protected mRNA analysis in colon epithelial cells and colon cancer cells. COPS7B was among the key genes that consistently showed both significant TE increase and protein elevation without transcriptional alteration in CRC. IGF2BP3 enhanced the TE of COPS7B mRNA to promote CRC growth and metastasis. COPS7B was found to be a component of the ribo-interactome that interacted with ribosomes to facilitate ribosome biogenesis and mRNA translation initiation. Collectively, this study revealed the proteomic features of CRC and highlighted elevated mRNA translation as a hallmark of CRC. The identification of the IGF2BP3-COPS7B axis underlying the increased protein synthesis rate in CRC provided a promising therapeutic target to treat this aggressive disease.

2023-08-07 | GSE240217 | GEO

IGF2BP3 controls cancer cell invasiveness by modulation RISC function

Project description:The goal of this study was to determine IGF2BP3 regulation of RNA targets in human pacreatic ductal adenocarcinoma cell lines Included are iCLIP-seq libraries for IGF2BP3 from PL45 and Panc1 PDAC cell samples, RIP-seq samples from PL45 and Panc1 PDAC cells, RNA-seq data sets from control and IGF2BP3 knockdown in PL45 and Panc1 PDAC cells, and small RNA-seq samples from Panc1 cells

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Glucose depletion inhibits translation initiation in yeast - transcription profiling

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Project description:Round spermatid injection (ROSI) gives rise to a lower birth rate than intracytoplasmic sperm injection (ICSI), which uses mature spermatozoa and has limited clinical application. Inefficient development of ROSI-embryos has been attributed to epigenetic abnormalities. However, the chromatin-based mechanism that underpins the low birth rate of ROSI remains to be determined. Here we show that a repressive histone mark H3K27me3 persists from doner round spermatids into zygotes in ROSI and that paternally derived H3K27me3 is associated with less accessible chromatin and impaired zygotic genome activation (ZGA) in ROSI-derived zygotes. These H3K27me3 marked loci in the paternal germline undergo histone turnover in spermiogenesis, resulting in the reduced paternal H3K27me3 in normal spermatozoa. Thus, the lack of histone turnover during spermiogenesis led to the dysregulation of chromatin accessibility and ZGA in ROSI-embryos. Our results unveil a molecular logic by which chromatin states in round spermatids impinge on chromatin states and ZGA in ROSI-embryos, highlighting the importance of chromatin remodeling in spermiogenesis in successful reproduction.

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Paternally inherited H3K27me3 impacts zygotic genome activation in round spermatid injection [ATAC-seq]

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The RNA binding protein IGF2BP3 promotes hematopoietic progenitor cell proliferation by targeting leukemogenic pathways

Project description:The goal of this study was to determine IGF2BP3 RNA targets in human B-cell Acute Lymphocitic Leukemia cell models. Included are iCLIP-seq libraries for IGF2BP3 from RS4;11 and REH B-ALL cell samples and RNA-seq data sets from control and IGF2BP3 knockdown RS4;11 B-ALL cell lines

2016-01-16 | E-GEOD-76931 | biostudies-arrayexpress

The IGF2BP3-COPS7B axis facilitates mRNA translation to drive colorectal cancer progression

Project description:The IGF2BP3-COPS7B axis facilitates mRNA translation to drive colorectal cancer progression

| PRJNA1002994 | ENA

Teleost spermatozoa can conduct de novo cytoplasmic and mitochondrial translation during post-testicular maturation

Project description:Translational silence of spermatozoa has long been considered the norm in animals. However, studies in mammals have shown that the mitochondrial ribosomal machinery is selectively activated during capacitation in the female reproductive tract, while cytoplasmic ribosomes remain inactive. Here, using quantitative proteomics in a piscine model species, we show that proteins involved in mRNA processing and cytoplasmic translation are predominantly accumulated in maturing spermatozoa within the extratesticular excurrent ducts, while those related to flagellar motility are enriched in mature (ejaculated) sperm. Based upon in vitro incubation of spermatozoa, motility assays and polysome profiling, we further show that 80S cytoplasmic and 55S mitochondrial ribosomes are actively involved in the translation of motility- and osmoadaptation-related proteins. These findings thus reveal that piscine spermatozoa can maintain de novo protein synthesis through both mitochondrial and cytoplasmic ribosomal activity during post-testicular maturation, which is necessary for the acquisition of full sperm function.

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