Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1080256 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Intratracheal instillation of quaternary ammonium compounds in rats
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Project description:Adaptation of Listeria monocytogenes EGD-e to quaternary ammonium compounds
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Project description:Overuse of cationic biocides including quaternary ammonium compounds (QACs) poses a growing threat by selecting for antimicrobial resistance. Here, we use Streptococcus gordonii, a commensal opportunistic pathogen, as a model to elucidate the mechanisms of QAC lethality and resistance. We show that QACs trigger V-type ATPase–driven metabolic dysfunction, leading to oxidative phosphorylation (OXPHOS) dominance and excessive reactive oxygen species (ROS) accumulation. Resistance arises through synergistic regulation by ClpX and PstB, which repress the competence pathway regulator ComDE and reprogram metabolism toward aerobic glycolysis, thereby limiting ROS production. Mechanistically, ClpX modulates ComDE signaling through protein aggregation and HtrA-mediated degradation of competence-stimulating peptide, while PstB governs ComDE via phosphorylation of the mannose-specific phosphotransferase system. Activation of ComDE restores ROS-mediated QAC lethality, identifying this pathway as a potential therapeutic target. These findings reveal that metabolic adaptation driven by competence regulon governs biocide susceptibility and adaptation, offering new strategies to counteract QAC resistance and associated cross-resistance in commensal bacteria.
2026-05-31 | GSE297304 | GEO
Project description:The goals of this study are to use SWATH-MS to detect bacterial proteomic profiles of wild-type Acinetobacter baylyi ADP1, and its protein response under the exposure of disinfectants, including chloramine and free chlorine. The concentrations of disinfectants were 10 mg/L. The group without dosing disinfectant was the control group. Each concentration was conducted in triplicate. By comparing the proteomic profiles of experimental groups and control group, the effects of disinfectants on translational levels can be revealed.
2021-09-09 | PXD016794 | Pride
Project description:Benzalkonium chlorides (BACs) are quaternary ammonium compounds widely used in various consumer products and industries due to their broad spectrum anti-antimicrobial properties. Their mechanism of action is proposed through the perturbation and disruption of a lipid membrane with its amphiphilic structure, in which BACs consist of a positively charged nitrogen head group and long aliphatic tail consisting of various lengths (Merchel Piovesan Pereira & Tagkopoulos, 2019). Because of this mechanism, they are used as active ingredients in disinfectant and cleaning solutions (sprays, wipes and hand sanitizers), medical products (eye drops and nasal sprays), cosmetic and hygiene products, and used as sanitization tools in the agricultural and food processing industries (Merchel Piovesan Pereira & Tagkopoulos, 2019). Detectable levels of BACs on various food products (The Federal Institute for Risk Assessment (BfR) of Germany, 2012) as well as topical exposure to BACs through disinfectant application to various surfaces and mediums can lead to chronic and systemic exposure to humans. Major routes of exposure include ingestion, inhalation and dermal/ocular/nasal contact (US EPA, 2006). The COVID-19 pandemic has led to a dramatic increase in the usage of cleaning and disinfectant solutions in businesses and households, increasing the amount of indoor exposure to these compounds (Zheng et al., 2020).
2026-06-23 | GSE302288 | GEO