Project description:We used microarrays to detail the global program of gene expression underlying the effect of p17 on human plasmacytoid dendritic cells and was compared to CpG profile. Experiment Overall Design: Human plasmacytoid dendritic cells were purified from two donors and cultured in the presence of p17 or CpG for 18 h.
Project description:We used microarrays to detail the global program of gene expression underlying the effect of p17 on human plasmacytoid dendritic cells and was compared to CpG profile. Keywords: gene expression study
Project description:To investigate the effect of IVIG and desialylated IVIG on the activation of plasmacytoid dendritic cells (pDCs). Human primary plasmacytoid dendritic cells were treated with TLR stimulation together with or without IVIG or desialylated IVIG, and the change of genes were analyzed. Primary human pDCs were preincubated with or without 10mg/ml IVIG or desialylated IVIG followed by stimulation with CpG overnight. The different genes between IVIG+CpG and desialylated IVIG+CpG were analyzed.
Project description:Human monocyte-derived dendritic cell cultures devoid of contaminating plasmacytoid dendritic cells were treated with the D-type CpG-oligonucleotide D19, with the non-CpG-oligonucleotide control D, or were left untreated. Cells of all treatment groups stemmed from the same donor. 6 h after addition, cells were harvested, and total RNA was prepared for analysis of D19-dependent differential gene expression on Affymetrix HG-U 133A 2.0. Keywords: agent response
