Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ScRNAseq of CD8+ T cells from autoimmune encephalitis

ABSTRACT: scRNAseq of CD8+ T cells from autoimmune encephalitis

PROVIDER: PRJNA1098728 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

scRNAseq of CD8+ T cells from autoimmune encephalitis

Project description:Single-cell RNA sequencing in seven anti-Ri autoimmune encephalitis (AIE) patients and three controls showed that neuron-reactive CD8+ T cells are cytotoxic KIR+ CD8+ regulatory T cells. In Ri-AIE, these cells had reduced KIR and IKZF2 (Helios) expression, alongside activated TCR signaling and elevated TNF and IFNG. They also overexpressed TOX, linked to brain-infiltrating cytotoxicity. These findings suggest a loss of regulatory function in KIR+ CD8+ T cells contributes to Ri-AIE pathogenesis.

2025-08-15 | GSE263666 | GEO

Proteomic analysis of meninges from autoimmune encephalitis (mouse model)

Project description:We developed a B-cell-mediated mouse model of Anti-N-Methyl-D-aspartate receptor autoimmune encephalitis (AE) by immunization with a GluN1359–378 peptide. In our model, mice display a series of symptoms that recapitulate autoimmune encephalitis such as anxiety behaviour and spatial memory impairment (Wagnon et al, Brain 2020) . Proteomic analysis of AE meninges showed enrichment of differentially expressed proteins in biological processes associated with B cell activation and cytokine signalling pathways.

2024-11-12 | PXD057817 |

scRNAseq of HLA-A*02:01-G5R18-26-specifi CD8+ T cells

Project description:In 2022, a global mpox outbreak occurred, and remains a concern today. The T cell memory response to MPXV infection has not been fully investigated. In this study, we evaluated this response in convalescent and MVA-BN vaccinated individuals using VACV-infected cells. Detailed phenotypic and scRNAseq analysis was focused on the immunodominant HLA-A*02:01-G5R18-26-specific CD8+ T cell response. T cells from convalescent individuals showed greater cytotoxicity, migratory potential to site of infection and TCR clonal expansion. Our study suggests a better functional profile of MPXV-specific memory T cells induced by natural infection, which may have an implication on the long-term protective responses to future infection.

2025-03-17 | E-MTAB-14244 | biostudies-arrayexpress

scRNAseq and TCRseq of tumor infiltrating CD8 T cells from samples

Project description:scRNAseq and TCRseq of tumor infiltrating CD8 T cells from samples

| PRJNA1289439 | ENA

Effect of Liraglutide on Experimental Autoimmune Encephalitis Mice Lumbar Spinal Cord

Project description:Effect of Liraglutide on Experimental Autoimmune Encephalitis Mice Lumbar Spinal Cord

| PRJNA773390 | ENA

Antitumor progenitor exhausted CD8 T cells are sustained with TCR engagement [scRNAseq]

Project description:Antitumor progenitor exhausted CD8 T cells are sustained with TCR engagement [scRNAseq]

| PRJNA1093430 | ENA

scRNAseq of HLA-A*02:01-G5R18-26-specifi CD8+ T cells

Project description:scRNAseq of HLA-A*02:01-G5R18-26-specifi CD8+ T cells

| PRJEB77832 | ENA

scRNAseq muscle stem cells

Project description:scRNAseq muscle stem cells

| PRJNA631918 | ENA

Targeting pathogenic CD8+ tissue-resident T cells with chimeric antigen receptor therapy in murine autoimmune cholangitis

Project description:Primary biliary cholangitis (PBC) is a cholestatic autoimmune liver disease secondary to an autoreactive T cell response against intrahepatic small bile ducts. Multiple murine models have demonstrated the critical role of effector CD8+T cells in this response and our work has used IL-12p40-/-IL-2Rα-/- mice (DKO mice) to study this issue. Herein we first demonstrated that use of either a CD8a knock-out or an anti-CD8a antibody prevents/reduces biliary immunopathology. Bulk and single-cell RNA sequencing analysis identifies CD8+ tissue resident memory T cells (Trm) in the liver of DKO mice, which highly express activation and cytotoxicity associated markers and have the ability to induce apoptosis of bile duct epithelial cells. Liver CD8+Trm cells also upregulate expression of several immune checkpoint molecules and in particular we identified PD-1 as a specific liver CD8+Trm cell marker in this model. Based on these data we constructed a novel chimeric antigen receptor containing a PD-L1 extracellular domain to target PD-1-expressing CD8+Trm cells. Importantly, treatment of DKO mice with PD-1 targeting CAR-T cells selectively depleted liver CD8+Trm cells in vivo and alleviated autoimmune cholangitis.

2023-09-05 | GSE241014 | GEO

PD-1+CD8+ T Cell-Mediated Hepatocyte Pyroptosis Promotes Progression of Murine Autoimmune Liver Disease

Project description:The specific mechanisms underlying effector pathways in autoimmune liver disease remain enigmatic and therefore constructing appropriate murine models to investigate disease pathogenesis becomes critical. A spontaneous severe murine model of autoimmune liver disease has been previously established in dnTGFβRII Aire-/- mice, exhibiting disease phenotypes that resemble both human primary biliary cholangitis (PBC) and autoimmune hepatitis (AIH). The data suggest that auto-reactive liver-specific CD8+ T cells are the primary pathogenic cells in liver injury. In this study, these data are advanced through the use of both single-cell sequencing and extensive in vitro analysis. The results identify a specific expanded pathogenic subset of PD-1+CD8+ T cells in the liver, exhibiting strong functional activity and cytotoxicity against target cells. Depletion of PD-1+CD8+ T cells using CAR-T cells effectively alleviates the disease. GSDMD-mediated pyroptosis is found to be aberrantly activated in the livers of model mice, and treatment with a GSDMD-specific inhibitor significantly inhibits disease progression. In vitro experiments reveal that PD-1+CD8+ T cells can induce the pyroptosis of hepatocytes through elevated production of granzyme B and perforin-1. These results provide a novel explanation for the cytotoxic activity of pathogenic liver PD-1+CD8+ T cells in autoimmune liver diseases and offer potential therapeutic targets.

2024-10-31 | GSE199065 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data