RIP-seq reveals the interactome of nucleolin in activated CD4+ T cells
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1099297 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR28637227_1.fastq.gz | Fastqsanger.gz | |||
| SRR28637227_2.fastq.gz | Fastqsanger.gz | |||
| SRR28637228_1.fastq.gz | Fastqsanger.gz | |||
| SRR28637228_2.fastq.gz | Fastqsanger.gz |
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Project description:During the actiation of CD4+T cells, the pri-miRNAs translocated from cytoplasma into nucleus.However, the mechanism remains unkown. We found that pri-miR-31 interact with nucleolin through G-guadruplex struction in pri-miR-31. We then transfected ASO to destribute the interaction of nucleolin and G4 struction. In order to identify the interacting RNA, including miRNA precuser and mRNA, we performed the RIP-seq Immunoprecipited by anti-nucleolin and IgG.
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Project description:Identification of the Early Vasoactive Intestinal Peptide (VIP) Transriptome and its Associated Interactome in Activated Murine CD4 T Cells In an attempt to understand the biological role of this neuropeptide in the immune system, we choose CD4 T cells as a cellular system for identifying a VIP-induced transcriptome. Murine CD4 T cells were isolated and used to identify changes in gene expression in the presence of PMA/ionomycin (activated) for five hours with and without 10-7 M VIP.
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