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Metabolic shifts during growth deceleration in Drosophila imaginal wing discs

ABSTRACT: Metabolic shifts during growth deceleration in Drosophila imaginal wing discs

PROVIDER: PRJNA1101014 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28709860_1.fastq.gz	Fastqsanger.gz
	SRR28709860_2.fastq.gz	Fastqsanger.gz
	SRR28709861_1.fastq.gz	Fastqsanger.gz
	SRR28709861_2.fastq.gz	Fastqsanger.gz
	SRR28709862_1.fastq.gz	Fastqsanger.gz

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Metabolic shifts during growth deceleration in Drosophila imaginal wing discs

Project description:Growth during development is often characterised by progressive deceleration, as organisms, tissues, and organs approach their final size. Using transcriptomics analysis, we show that, during their two-day period of growth deceleration, wing imaginal discs of Drosophila undergo a progressive metabolic shift away from oxidative phosphorylation and towards glycolysis. We then develop a sensitive reporter of HIF-1a activity (a proxy for hypoxia), which allows us to show that imaginal discs become mildly, though increasingly, hypoxic during normal development, in normoxic conditions. As a result, HIF-1a dampens growth via transcriptional activation of scylla/REDD1, a negative regulator of TOR signalling. We find that overactivation of TOR signalling triggers a marked hypoxia response both cell-autonomously and in surrounding tissues. This suggests that, in wildtype animals, TOR activity imposes increasing pressure on oxygen supplies. Indeed, in the absence of HIF-1a, TOR signalling triggers JNK signalling, a marker of cellular stress. Moreover, TOR-induced stress is alleviated by a small increase is oxygen supplies. Our results suggest that, during normal development, Sima/HIF-1α prevents growth-induced demand from exceeding available supplies, which become increasing scarce as tissue size increases.

2025-04-16 | GSE264119 | GEO

SmD3 protein interactome in Drosophila melanogaster wing imaginal discs

Project description:Screening for binding partners of the splicing factor SmD3 and changes in interaction upon depletion of the protein Ecdysoneless (Ecd) in the nubbin domain of third-instar larval wing imaginal discs.

2021-09-09 | PXD019761 | Pride

Single-cell transcriptomic analysis of the scrib mutant wing imaginal discs

Project description:Investigation of intratumor heterogeneity in the scrib¹ mutant wing imaginal discs. Method: Staged scrib¹ wing imaginal discs were dissected and transferred to DPBS. The wing imaginal discs were dissociated in 0.25% Trypsin-EDTA solution at 37 ℃ for 10 min. Cells were then washed in DPBS and passed through 35μm filter before library preparation. Construction of 10x single cell libraries and sequencing on Illumina platform were performed by Novogene.

2019-08-27 | GSE130566 | GEO

RNA-seq of Drosophila melanogaster wing imaginal discs during L3 stage

Project description:During the last larval stage (L3) of Drosophila melanosgaster development, wing imaginal discs undergo tremendous growth and initiate differentiation programs. This transcriptomic time course aims at uncovering the the genes that are temporally regulated during the L3 in wing imaginal discs to regulate the dynamics of growth and differentiation.

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Transcriptome analysis of dNKAP knockdown and control wing imaginal discs

Project description:We report the genome-wide profiling of wing imaginal discs upon dNKAP knockdown

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Single-cell transcriptomic analysis of 96hour AEL wild type wing imaginal discs

Project description:Genotype: FRT82B Method: Staged wild type wing imaginal discs were dissected and transferred to DPBS. The wing imaginal discs were dissociated in 0.25% Trypsin-EDTA solution at 37 ℃ for 10 min. Cells were then washed in DPBS and passed through 35μm filter before library preparation. Construction of 10x single cell libraries and sequencing on Illumina Hiseq platform were performed by Novogene.

2019-08-31 | GSE133204 | GEO

Gene expression profile of Drosophila third instar larval wing imaginal discs overexpressing netrinA (netA) and frazzled (fra).

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Comparison of ATAC seq between control wing imaginal discs and salm/salr knockdown wing imaginal discs

Project description:Comparison of ATAC seq between control wing imaginal discs and salm/salr knockdown wing imaginal discs

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Gene expression profile of Drosophila third instar larval wing imaginal discs overexpressing netrinA (netA) and frazzled (fra).

Project description:In order to analyze the global changes in gene expression resulting from induction of NetA-Fra signaling, we carried out a microarray experiment comparing Drosophila third instar wing imaginal discs in which Net+Fra had been overexpressed to age matched wild type wing imaginal discs. RNA extracted from both +NetA-Fra overexpression and wildtype third instar imaginal discs were hybridized to the Affymetrix GeneChip Drosophila Genome 2.0 . Heat shock induced GFP-marked clones ectopically expressing NetA+Fra in larvae were generated. Controls for this study included age matched wildtype third instar wing imaginal discs bearing GFP clones which were prepared in the same manner. Total RNA was extracted from dissected +NetA-Fra vs. control third instar wing imaginal discs and hybridized to the Affymetrix GeneChip Drosophila Genome 2.0.

2013-05-21 | E-GEOD-47113 | biostudies-arrayexpress

Indentification of Dis3L2 targets in Drosophila melanogaster wing imaginal discs

Project description:We investigated the effect on mRNA expression in Drosophila melanogaster wing imaginal discs following the knockdown of the 3'-5' exoribonuclease Dis3L2.

2016-11-16 | E-MTAB-4545 | biostudies-arrayexpress

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