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Conduritol B Epoxide treatment of MSCs and 293T cells

ABSTRACT: Conduritol B Epoxide treatment of MSCs and 293T cells

OTHER RELATED OMICS DATASETS IN: GSE13675

PROVIDER: PRJNA110421 | ENA |

REPOSITORIES: ENA

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Conduritol B Epoxide treatment of MSCs and 293T cells

Project description:The purpose of this study was to study the biological consequences of inhibiting acid beta-galactosidase with the use of conduritol b epoxide, which serves as a chemical model of Gaucher disease

2008-12-15 | GSE13675 | GEO

ATO treatment on mesenchymal stem cells (MSCs) interacting breast cancer cells

Project description:ATO treatment on mesenchymal stem cells (MSCs) interacting breast cancer cells

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ATO treatment on mesenchymal stem cells (MSCs) interacting breast cancer cells

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HEK 293T LCMS after 8h of treatment with Actinomycin D

Project description:Investigation of proteomic changes in HEK 293T cells after 8h of actinomycin D treatment with special focus on RNA modifying enzymes.

2023-07-20 | PXD039549 | Pride

RNA-seq analysis of miR-210 KO 293T cells

Project description:To study the effects of miR--210 on 293T cells and hypoxia response, we used the CRISPR/Cas9 system to knockout (KO) the human miR-210 gene in 293T cells. We then added DMOG to mimic hypoxia condition and analyzed the RNA expression profile of wildtype (WT) and KO cells under normal and hypoxic conditions. DMOG treatment and RNA-seq were performed three times (experiment 1, 2, and 3).

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Inflammatory cytokines pre-treatment enhances the immunomodulatory property of hUC-MSCs

Project description:Mesenchymal stem cells (MSCs) exist in almost all tissues and participate in tissue regeneration and homeostasis. MSCs based therapy is applied to some refractory immune diseases to control inflammation, such as lupus nephritis, Crohn’s disease and rheumatoid arthritis. However, accumulating studies showed that the immunomodulatory capacity of naïve MSCs is mild and limited. To enhance MSCs immunomodulatory function, researchers innovated a new method to reprogram MSCs via pre-treatment with inflammatory cytokines. In this work, we firstly used a cocktail of three cytokines, IL-1β, TNF-a and IFN-γ, to treat hUC-MSCs (human MSCs from umbilical cord). We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 untreated hUC-MSCs (ctrl) and 3 cytokines-treated hUC-MSCs (primed).

2023-06-12 | GSE224190 | GEO

MSCs-encapsulated porous microparticle eye drops for autoimmune dry eye disease treatment

Project description:Dry eye disease (DED) characterizes by chronic inflammation and an unstable tear film. Stem cells have shown potential for DED treatment, but the main challenge lies in improving the effectiveness of cell delivery. Here, we developed novel eye drops for DED treatment by employing porous microcarriers with mesenchymal stem cells. The microcarriers were created by electrospraying the solution of Arginine-Glycine-Aspartic Acid (RGD) peptides-modified sodium alginate with polyethylene oxide and mesenchymal stem/stromal cells (MSCs) into the calcium chloride solution. In vitro experiments based on a hyperosmotic corneal epithelial cell model indicated that porous microcarriers encapsulated with MSCs (RGD-Alg@MSCs) demonstrated notable enhancements in cell viability, reductions in apoptosis and reactive oxygen species (ROS), and diminished expression of pro-inflammatory cytokines. In the DED model using non-obese diabetic (NOD) mice, RGD-Alg@MSCs effectively enhanced tear production, promoted corneal healing, and alleviated inflammation. Additionally, RGD-Alg@MSCs modulated the immune environment in DED by inhibiting dendritic cell (DC) activation and suppressing Th17 differentiation in vitro, effectively disrupting the inflammatory feedback loop characteristic of DED. This immune modulation strategy was further validated through in vivo experiments, confirming its therapeutic potential. Thus, the designed MSCs-encapsulated porous microcarrier system can improve stem cell delivery and DED treatment efficiency.

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RNA binding profile of MED23 in 293T cells

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