Project description:We report the outcomes of next-generation sequencing (RNA-Seq) of guinea pig (Cavia porcellus) heart tissue and compare relative transcript abundances between fetus and adult.
Project description:Preimplantation development is a pivotal phase in human embryogenesis, establishing fundamental lineages and being crucial for overall health. Ethical constraints with human embryos necessitate a model organism, and the guinea pig, with its physiological similarities to humans, emerges as a valuable alternative. This rodent mirrors human preimplantation timing, placental features, and fetal development. Importantly, it serves as a model for studying long-term consequences of prenatal insults. Comparative studies across species, including guinea pigs, are essential for unraveling pluripotency mechanisms, offering insights into conserved and divergent aspects and understanding evolutionary adaptations. Addressing the lack of guinea pig embryonic transcriptomic data, this research employs single-cell RNA sequencing, immunofluorescence, and epigenetic analyses to explore gene expression, metabolic requirements, and X-chromosome inactivation dynamics. The study advances our understanding of early embryogenesis, emphasizing the guinea pig's relevance as a model for developmental and pluripotency research.
Project description:We report the transcriptome profiles of guinea pig cytomegalovirus (Caviid betaherpesvirus 2 ; GPCMV) infected and uninfected cells. RNA was harvested at an early time post-infection. Data sets from a lung fibroblast cell line (JH4), primary amnion derived cells (1° AECs), and HPV16 E6/E7-transduced amniotic epithelial cells (AECD) is shared.
Project description:Proteomic analyses of the ocular posterior pole tissues at 6 weeks after induction of form-deprived myopia and lens-induced myopia models in guinea pigs.
Project description:To investigate the effect of the dexamethasone-eluting electrode in the guinea pig cochlea, and compared the gene expression after 7 days insertion with that of a normal electrode or non-treated control by microarray. Male Hartley guinea pigs (SLC, Shizuoka, Japan) with an age of seven weeks were used for the study. Three were implanted with normal electrodes while three others received a dexamethasone-eluting electrode. The cochleae from two animals, which did not undergo surgery. Seven days after electrode implantation the whole temporal bone was removed and placed into RNAlater solution (Ambion, Life Technologies Co., Grand Island, NY) to stabilize and protect cellular RNA. The whole cochlea was dissected out under a microscope and total RNA were extracted.
Project description:RNA was extracted from the meninges of mice from either Specific pathogen free or Germ free facilities or from the offspring of mice reconstituted with different human microbiomes.