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Transcriptome profiling of ANXA4 KO in A498 cells

ABSTRACT: Transcriptome profiling of ANXA4 KO in A498 cells

PROVIDER: PRJNA1112795 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29074346_1.fastq.gz	Fastqsanger.gz
	SRR29074346_2.fastq.gz	Fastqsanger.gz
	SRR29074347_1.fastq.gz	Fastqsanger.gz
	SRR29074347_2.fastq.gz	Fastqsanger.gz
	SRR29074348_1.fastq.gz	Fastqsanger.gz

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Transcriptome profiling of ANXA4 KO in A498 cells

Project description:ANXA4 WT (control) and ANXA4 KO in A498 kidney cancer cells was generated using CRISPR/Cas9 and two independent gRNAs for control and two independent gRNAs targeting ANXA4. Single cells clones (Ctrl.-1, Crtl.-2, KO-1, KO-2) were selected and validated by western blotting confirming loss of ANXA4 in KO cell lines on protein level. Transcriptome profiling (RNA-Sequencing) and differential gene expression analysis of 2 independent replicates per cell line was performed (resulting in 4 control and 4 ANXA4 KO sequencings). ANXA4 KO cells exhibit transcriptional changes compared to WT cells including upregulation of the ETS transcription factor ELF3 and regulation of Matrisome genes.

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Project description:A498 cells, established from a clear cell renal cell cancer of a 52 years old male patient, were obtained from ATCC and maintained in DMEM supplemented with 10% fetal calf serum (FCS). This cell line does not express functional VHL. A498 subclones stably transfected with HA-pVHL30 or control plasmid have been described and were maintained in 0.5 mg/ml G418.

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