Project description:Duchenne muscular dystrophy (DMD) is caused by genetic deficiency of dystrophin and characterized by massive structural and functional changes of skeletal muscle tissue, leading to terminal muscle failure. In this project, proteomics data from skeletal muscle of a genetically engineered DMD pig model were investigated in order to confirm muscular fibrosis and MSOT signals.

2020-01-09 | PXD016003 | Pride

Three-dimensional tissue-engineered human skeletal muscle model of satellite cell quiescence

Project description:In vivo, satellite cells (SCs) are essential for skeletal muscle repair. However, in vitro investigation of SC function is challenged by isolation-induced SC activation, loss of the native quiescent state, and differentiation to myoblasts. This study applies tissue-engineered human skeletal muscle to track myoblast deactivation to 3D-SCs, which bear a quiescent phenotype, as well as examine melittin-induced injury response.

2021-11-11 | GSE188215 | GEO

Human engineered skeletal muscle of hypaxial origin from pluripotent stem cells with advanced function and regenerative capacity

Project description:Human pluripotent stem cell derived muscle models show great potential for translational research. Here, we describe novel developmentally inspired methods for the derivation of skeletal muscle cells and their utility in three-dimensional skeletal muscle organoid formation as well as skeletal muscle tissue engineering. Three-dimensional organoid and tissue engineered models exhibit organotypic maturation and function and regenerative responses, recapitulating canonical properties of bona fide skeletal muscle in vivo. Key steps include the directed differentiation of human pluripotent stem cells to embryonic muscle progenitors of hypaxial origin followed by primary and secondary fetal myogenesis with development of a satellite cell pool and evidence for innervation in vitro. Regenerative competency was demonstrated in a cardiotoxin injury model with evidence for satellite cell activation as underlying mechanism.

2022-10-19 | GSE178270 | GEO

Effects of electrical pulse stimulation and hydrogen peroxide as its byproduct on in vitro contractile skeletal muscle tissue.

Project description:The in vitro human skeletal muscle model is a valuable tool for studying muscle physiology and the mechanisms underlying muscle diseases. Tissue engineering approach could produce contractile human muscle tissue, with electrical pulse stimulation (EPS) enhancing its contractile ability. We evaluated transcriptomic changes induced by EPS, two different patterns of EPS were applied to differentiated human myofiber sheets with cultured fibrin-gel. After 3 days of EPS-induced exercise, RNA was extracted from these two differently stimulated samples and non-stimulated control sample and analyzed by RNA-sequencing. For the samples with an excess EPS pattern, catalase was supplemented in the media to degrade hydrogen peroxide generated by EPS, and RNA was extracted from the stress-mitigated sample and also analyzed by RNA-sequencing.

2025-03-12 | GSE289180 | GEO

Proteome analysis of skeletal muscle samples from a porcine model of Duchenne muscular dystrophy treated by somatic gene editing

2020-02-05 | PXD014893 | Pride

Skeletal Muscle Transcriptomics

Project description:Skeletal Muscle Transcriptomics

| PRJNA532382 | ENA