Project description:The changes in the trabecular meshwork in steroid-induced glaucoma are similar to those in human primary open-angle glaucoma. To explore the changes in the trabecular meshwork in POAG, we extracted RNA from human trabecular meshwork cells with or without dexamethasone, followed by next-generation transcriptome sequencing to observe changes in gene expression in trabecular meshwork cells, thereby better understanding the mechanism of increased IOP.
Project description:Aqueous humor drainage through the trabecular meshwork is regulated in part by hormonal cues, including norepinephrine (NE) and dexamethasone (DEX), and its circadian variation is thought to reflect rhythmic changes in these signals. However, the transcriptional programs and molecular pathways through which NE and DEX influence human trabecular meshwork cells remain incompletely defined. In this study, we performed RNA-seq analysis of immortalized human primary trabecular meshwork cells (abm; T0371) exposed to NE or DEX for 6 hours, with vehicle-treated cells as controls, to identify stimulus-responsive genes and pathways associated with trabecular meshwork function and aqueous humor outflow.
Project description:Glucocorticoids with different chemical structures but similar glucocorticoid receptor potency regulate subsets of common and unique genes in human trabecular meshwork cells. Gene expression changes of human trabecular meshwork cells, TM 86 and TM 93, due to treatment with dexamethasone (Dex), fluocinolone acetonide (FA), and triamcinolone acetonide (TA).
