Transcriptomics,Genomics

Dataset Information

23

Effect of glucocorticoid chemical structure and receptor potency on human trabecular meshwork cells


ABSTRACT: Glucocorticoids with different chemical structures but similar glucocorticoid receptor potency regulate subsets of common and unique genes in human trabecular meshwork cells. Gene expression changes of human trabecular meshwork cells, TM 86 and TM 93, due to treatment with dexamethasone (Dex), fluocinolone acetonide (FA), and triamcinolone acetonide (TA). Overall design: Agilent Human Whole Genome Expression oligonucleotide microarray. Changes of genome-wide mRNA transcription levels were determined by treating cultured TM 86 and TM 93 cells with 1 uM of either Dex, FA, and TA for 24 hours versus DMSO vehicle controls. This was followed by total RNA extraction, labeling of RNA with Cy3, and hybridization of Cy3-labeled cRNA to the Agilent Human Whole Genome microarray for 17 hours. The microarray experiment was repeated three times.

INSTRUMENT(S): Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)

SUBMITTER: Alissar Nehme   

PROVIDER: GSE16643 | GEO | 2010-02-15

SECONDARY ACCESSION(S): PRJNA116225

REPOSITORIES: GEO

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Publications

Glucocorticoids with different chemical structures but similar glucocorticoid receptor potency regulate subsets of common and unique genes in human trabecular meshwork cells.

Nehmé Alissar A   Lobenhofer Edward K EK   Stamer W Daniel WD   Edelman Jeffrey L JL  

BMC medical genomics 20090910


BACKGROUND: In addition to their well-documented ocular therapeutic effects, glucocorticoids (GCs) can cause sight-threatening side-effects including ocular hypertension presumably via morphological and biochemical changes in trabecular meshwork (TM) cells. In the present study, we directly compared the glucocorticoid receptor (GR) potency for dexamethasone (DEX), fluocinolone acetonide (FA) and triamcinolone acetonide (TA), examined the expression of known GRalpha and GRbeta isoforms, and used  ...[more]

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