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RNA transcripts regulate G-quadruplex landscapes through G-loop formation (RNA-Seq)

ABSTRACT: RNA transcripts regulate G-quadruplex landscapes through G-loop formation (RNA-Seq)

PROVIDER: PRJNA1120076 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29283100.fastq.gz	Fastqsanger.gz
	SRR29283101.fastq.gz	Fastqsanger.gz
	SRR29283102.fastq.gz	Fastqsanger.gz
	SRR29283091.fastq.gz	Fastqsanger.gz
	SRR29283092.fastq.gz	Fastqsanger.gz

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RNA transcripts regulate G-quadruplex landscapes through G-loop formation (RNA-Seq)

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Project description:G-quadruplexes (G4s) are epigenetic DNA structures that regulate transcription but also threaten genome stability when dysregulated. It remains unknown how G4 dynamics is organized in a cell-specific manner. Here, we report that RNA transcripts govern G4 landscapes through homology-directed invasion opposite the G4 strand, forming ‘G-loops’. G-loops are disassembled via DHX36-FANCJ-mediated G4 unwinding and subsequent nucleolytic incision by XPF-ERCC1, leading to hybrid strand renewal through DNA synthesis. G-loop disassembly requires stochiometric invading RNAs, by contrast, excess of which stabilizes G-loops against disassembly. Defects in G-loop disassembly accumulate G-loops predominantly in gene regulatory regions, leading to transcriptional dysregulation, replication stress, and DNA damage throughout the cell cycle. These findings establish nuclear RNAs as pivotal G-loop regulators, linking the transcriptome to gene regulation and genome maintenance.

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