Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

U937 cells: Control treated LPS vs. Co-cultured Panc-1 cells

ABSTRACT: U937 cells: Control treated LPS vs. Co-cultured Panc-1 cells

PROVIDER: PRJNA1123739 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

U937 cells: Control treated LPS vs. Co-cultured Panc-1 cells

Project description:We collected U937 cells after 72 h of LPS treatment or 72 h of co-culture with PANC-1 cells for microarray analysis. The gene microarray data were analyzed using the Agilent Human whole genome oligo 4*44k chip (array kit serial number: US00082833) and Agilent’s gene expression microarray software, including Feature Extraction and GeneSpring GX. Feature Extraction software extracts data from microarray images and provides workflows for analysis. GeneSpring GX offers statistical tools for data analysis and visualization (Welgene Biotech), and the genes of interest were drawn as a heatmap.

2024-06-14 | GSE269815 | GEO

Real-time quantitative PCR analysis of differentiated U937 cells stimulated with LPS+IFNgamma

Project description:U937 monocyte cell line was differentiated using PMA. Differentiated U937 monocytes were exposed to MEM+10% FBS medium (untreated cells) and the same medium containing LPS+IFNgamma. The changes in the gene expression of cytokines and chemokines were evaluated using RT2 Profiler qPCR array

2024-08-23 | GSE275490 | GEO

U937 cells: differentiating vs. undifferentiated

Project description:Gene-expression changes in U937 cells undergoing monocytic differentiation with TPA treatment Keywords: differentiation treatment

2008-08-29 | GSE10560 | GEO

RNA sequencing of Panc-1 cells co-cultured with CAF overexpressing PAI-1

Project description:To investigate the effect of CAF overexpressing PAI-1 on tumor cells, CAF overexpressing PAI-1 was used to co-culture with Panc-1 cells, and then gene expression profiling of Panc-1 cells was performed using RNA-seq.

2026-03-29 | GSE278295 | GEO

U937 ctrl vs U937 Saha (Vorinostat; suberoylanilide hydroxamic acid) 6h

Project description:miRNA expression profiling of U937 ctrl vs U937 treated Saha 5 µM 6h

2017-05-22 | GSE69957 | GEO

Real-time quantitative PCR analysis of differentiated U937 cells stimulated with LPS+IFNgamma

Project description:Real-time quantitative PCR analysis of differentiated U937 cells stimulated with LPS+IFNgamma

| PRJNA1151083 | ENA

U937 cells: differentiating vs. undifferentiated

Project description:Gene-expression changes in U937 cells undergoing monocytic differentiation with TPA treatment Keywords: differentiation treatment The promonocytic U937 cell line was maintained at 37C in RPMI 1640 medium (Mediatech) supplemented with 10% fetalclone (HyClone) under a 5% CO2, 95% air atmosphere. U937 cells were differentiated by the addition to the growth medium 100 nM 12-O-tetradeconoyl-phorbol 13-acetate (TPA) (Sigma).Three condition experiment, treated with TPA for two different time points (27 and 96 hrs) vs. untreated. Biological replicates: independently grown, treated and harvested

2010-06-20 | E-GEOD-10560 | biostudies-arrayexpress

50:50 mixture of U937 and K562 cells treated with 0.5μg/ml of LPS, profiled with ChronoSeq for 12 samples barcoded with unique Time-Tags

Project description:The intent of the experiment was to observe the response of K562 cells when stimulated with LPS. To show that K562 cells cannot respond to LPS either alone or when mixed with cells unresponsive to LPS; we LPS stimulated a 50:50 mixture of K562 and undifferentiated U937 cells which are known to show limited or no response to LPS. Cells were stimulated with 0.5ug/ml of LPS with a sampling interval of 10 minutes.

2025-11-01 | E-MTAB-15927 | biostudies-arrayexpress

U937 scramble (Usc) vs U937 miR-194-5p (UmiR-194-5p)

Project description:Transcriptional profiling of U937 scramble vs U937 miR-194-5p (UmiR-194-5p)

2017-05-22 | GSE73646 | GEO

B27 modulation of LPS response

Project description:Objectives How HLA-B27 contributes towards arthritis susceptibility is still unclear, but effects on the response to bacteria unrelated to the classical antigen presenting role of B27 have been suggested. This study investigated whether HLA-B27 modulates the innate response to LPS, a component shared between all Gram –ve bacteria that can trigger reactive arthritis. Methods Pools of U937 transfectants expressing either HLA-B27, HLA-A2, or the expression plasmid alone were differentiated with PMA and stimulated with LPS. Supernatants were analysed for TNF-alpha secretion and the gene expression profiles of unstimulated and LPS stimulated cells were determined by microarray analysis. Changes in gene expression that are indicative of an unfolded protein response were also analysed by quantitative PCR. Results TNF-alpha secretion, a biological marker of the inflammatory response to LPS, was not significantly different between U937-B27 and U937-control. No differences in gene expression between unstimulated U937- B27 and U937-control lines were detected. Both U937-control and U937-B27 exhibited a stereotypic response to LPS. Only 1 gene, OAS2, was differentially expressed by these cell lines, and this was confirmed by quantitative PCR. Analysis of XBP-1 splicing suggested that a small increase in the unfolded protein response is induced following LPS stimulation, but this increase was seen in all transfectants. Conclusions The expression of B27 does not profoundly alter gene expression following LPS stimulation. Therefore, additional signals, such as those provided by cytokines or intracellular infection, may be required to reveal any influence of B27 expression on the inflammatory response. Keywords: antigen response

2006-04-10 | GSE4579 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data