Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA112899 | ENA |
REPOSITORIES: ENA
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Project description:The mouse aldehyde oxidase, Aoh2 (aldehyde oxidase homolog 2), is a molybdo-flavoenzyme. Harderian glands are the richest source of Aoh2, although the protein is detectable also in sebaceous glands, epidermis and other keratinized epithelia. The levels of Aoh2 in the Harderian gland and skin are controlled by genetic background, being maximal in CD1 or C57BL/6 and minimal in DBA/2, CBA or 129/Sv strains. Testosterone is a negative regulator of Aoh2 in Harderian glands. Homogenously purified Aoh2 oxidizes retinaldehyde into retinoic acid efficiently, while it is devoid of pyridoxal oxidizing activity, unlike other aldehyde oxidases. Aoh2 knockout mice are viable and fertile, although they have an absolute deficit of retinaldehyde oxidase activity in the Harderian gland, decreased retinoic acid levels, and down-regulation of retinoid-dependent genes. The Harderian gland’s transcriptome of knockout mice is characterized by perturbations in pathways controlling lipid homeostasis and cellular secretion. This is particularly evident in sexually immature animals. Lower amounts of retinoic acid are also observed in the skin of Aoh2-/- animals. Knockout mice have thickening of the epidermis in basal conditions and after UV light exposure. This has correlates in the skin transcriptome, which shows enrichment and overall up-regulation of genes involved in hypertrophic responses. Keywords: Gene knockout study
2009-01-01 | GSE12541 | GEO
Project description: Not available
| S-EPMC8784167 | biostudies-literature
Project description:Gene expression by Retinoic Acid in mouse Dendritic Cells
| PRJNA151481 | ENA
Project description:RNA-seq analysis of retinoic acid treated mouse neuromesodermal progenitors
| PRJNA325436 | ENA
Project description:Lipid metabolism is recognized as a key process for stem cell maintenance and differentiation but genetic factors that instruct stem cell function by influencing lipid metabolism remain to be delineated. Here we identify Tnfaip2 as an inhibitor of reprogramming of mouse fibroblasts into induced pluripotent stem cells. Tnfaip2 knockout embryonic stem cells (ESCs) exhibit differentiation failure and knockdown of the planarian orthologue, Smed-exoc3, abrogates in vivo differentiation of somatic stem cells, tissue homeostasis, and regeneration. Tnfaip2 deficient ESCs fail to induce synthesis of cellular triacylglycerol (TAG) and lipid droplets (LD) coinciding with reduced expression of Vimentin (Vim) – a known inducer of LD formation. Knockdown of Vim and Tnfaip2 act epistatically in enhancing cellular reprogramming of mouse fibroblasts. Similarly, planarians devoid of Smed-exoc3 displayed acute loss of TAGs. Supplementation of palmitic acid (PA) and palmitoyl-L-carnitine (a mitochondrial carrier of PA) restores the differentiation capacity of Tnfaip2 deficient ESCs as well as stem cell differentiation and organ maintenance in Smed-exoc3-depleted planarians. Together, these results identify a novel pathway, which is essential for stem cell differentiation and organ maintenance by instructing lipid metabolism.
2020-11-16 | GSE134301 | GEO
Project description:Lipid metabolism is recognized as a key process for stem cell maintenance and differentiation but genetic factors that instruct stem cell function by influencing lipid metabolism remain to be delineated. Here we identify Tnfaip2 as an inhibitor of reprogramming of mouse fibroblasts into induced pluripotent stem cells. Tnfaip2 knockout embryonic stem cells (ESCs) exhibit differentiation failure and knockdown of the planarian orthologue, Smed-exoc3, abrogates in vivo differentiation of somatic stem cells, tissue homeostasis, and regeneration. Tnfaip2 deficient ESCs fail to induce synthesis of cellular triacylglycerol (TAG) and lipid droplets (LD) coinciding with reduced expression of Vimentin (Vim) – a known inducer of LD formation. Knockdown of Vim and Tnfaip2 act epistatically in enhancing cellular reprogramming of mouse fibroblasts. Similarly, planarians devoid of Smed-exoc3 displayed acute loss of TAGs. Supplementation of palmitic acid (PA) and palmitoyl-L-carnitine (a mitochondrial carrier of PA) restores the differentiation capacity of Tnfaip2 deficient ESCs as well as stem cell differentiation and organ maintenance in Smed-exoc3-depleted planarians. Together, these results identify a novel pathway, which is essential for stem cell differentiation and organ maintenance by instructing lipid metabolism.
2020-11-16 | GSE148758 | GEO
Project description: Not available
| S-EPMC11335981 | biostudies-literature
Project description:The mouse aldehyde oxidase, Aox4 (aldehyde oxidase 4), is a molybdo-flavoenzyme. Harderian glands are the richest source of Aox4, although the protein is detectable also in sebaceous glands, epidermis and other keratinized epithelia. We performed whole genome gene expression experiment on Harderian Gland, White Adipose Tissue and Liver of WT and Aox4-/- animals.
2015-09-15 | E-MTAB-3820 | biostudies-arrayexpress
Project description:PURPOSE: To provide a detailed gene expression profile of the normal postnatal mouse cornea. METHODS: Serial analysis of gene expression (SAGE) was performed on postnatal day (PN)9 and adult mouse (6 week) total corneas. The expression of selected genes was analyzed by in situ hybridization. RESULTS: A total of 64,272 PN9 and 62,206 adult tags were sequenced. Mouse corneal transcriptomes are composed of at least 19,544 and 18,509 unique mRNAs, respectively. One third of the unique tags were expressed at both stages, whereas a third was identified exclusively in PN9 or adult corneas. Three hundred thirty-four PN9 and 339 adult tags were enriched more than fivefold over other published nonocular libraries. Abundant transcripts were associated with metabolic functions, redox activities, and barrier integrity. Three members of the Ly-6/uPAR family whose functions are unknown in the cornea constitute more than 1% of the total mRNA. Aquaporin 5, epithelial membrane protein and glutathione-S-transferase (GST) omega-1, and GST alpha-4 mRNAs were preferentially expressed in distinct corneal epithelial layers, providing new markers for stratification. More than 200 tags were differentially expressed, of which 25 mediate transcription. CONCLUSIONS: In addition to providing a detailed profile of expressed genes in the PN9 and mature mouse cornea, the present SAGE data demonstrate dynamic changes in gene expression after eye opening and provide new probes for exploring corneal epithelial cell stratification, development, and function and for exploring the intricate relationship between programmed and environmentally induced gene expression in the cornea. Keywords: other
2004-01-02 | GSE887 | GEO