Project description:To clarify the pathological significance of CGRP in ulcerative colitis, we generated knockout mice for CGRPα and CGRPβ and analyzed colon proteome data from DDS drinking water ulcerative colitis model mice. In addition, to confirm changes in the colon over time, the colon of wild-type mice after DDS drinking was harvested over time and used for proteome data.
Project description:<p> This study investigated the therapeutic effects of FST on colitis in mice by exploring its impact on the gut microbiota, metabolites, and immune response. The results show FST alleviated colitis in mice, including mitigated weight loss, improved colon length shortening, and reduced mRNA expression of inflammatory factors (IL-6, TNF-α, IL-1β), while restoring the disrupted Th17/Treg balance in mesenteric lymph nodes, spleen, and colon tissues. 16S rRNA sequencing revealed gut microbiota dysbiosis in colitis mice, characterized by reduced abundance of Bacteroidota and Bifidobacterium. FST treatment markedly restored the balance among these genera, improving the gut microbiota dysbiosis. Particularly, FST enriched Bifidobacterium in colitis mice. Untargeted metabolomics analysis indicated FST altered downstream metabolite expression of Bifidobacterium, reducing LA level and increasing CLA level. RNA-seq revealed that CLA can regulate the PI3K-AKT signaling pathway. CLA inhibits the phosphorylation of PI3K, AKT, and mTOR proteins, thereby suppressing Th17 cells differentiation and promoting Treg cells differentiation. </p>
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the effects of macrophage-specific E4BP4 overexpression under c-fms gene promoter on colon macrophages during the recovery phase of Dextran Sodium Sulfate (DSS)-induced colitis. Methods: We generated transgenic mice (TG) with macrophage-specific E4BP4 overexpression. Colon macrophages were isolated at Zeitgeber Time (ZT) 0 from TG mice and WT littermates and total RNA was extracted. Colon macrophage RNA profiles were generated by deep sequencing for two groups with three mouse samples each. Results: There were significant differences between TG and WT mice. Conclusions: Colon macrophages from the E4BP4 transgenic mice during the recovery phase of DSS-induced colitis might be altered at the transcription level.
Project description:This study aims to investigate the protein expression profiles in a murine model of dextran sulfate sodium (DSS)-induced colitis using advanced Astral-DIA quantitative proteomics technology. A total of 12 colon tissue samples were analyzed, including 6 from healthy control mice and 6 from DSS-treated mice with induced colitis. Experimental Design Species: Mus musculus (C57BL/6 strain). Tissue Source: Colon tissues were dissected, snap-frozen in liquid nitrogen, and homogenized to extract proteins. Groups: Control Group: Healthy mice without intervention. DSS Group: Mice subjected to 2.5% DSS administration for 7 days to induce colitis, validated by histopathological assessment.
Project description:To investigate cell type-specific differentially expressed genes (DEGs) in the colon before and after the establishment of colitis in mice, mice were administered 3% DSS in distilled water or vehicle water for 7 days. Three mice were included in each group. On day 7, mice were sacrificed, and colon tissues were harvested for single-cell RNA sequencing (scRNA-seq).
Project description:To obtain insight into the complex traits underlying the local pathophysiological response to the repeated colitis inductions with TNBS, whole genome gene expression was performed on RNA isolated from the distal colon tissue of 5 mice/timepoint Total RNA obtained from isolated from the distal colon of TNBS colitis mice (isolated on day 9, 14, 16, 21, 23, and 28) was compared to those healthy control mice
Project description:This study investigates the therapeutic effects of RJA (purity 99.42%) on DSS-induced colitis in male C57BL/6J mice. Using high-throughput miRNA sequencing, we analyzed the miRNA expression profiles in mouse colon samples. The study revealed significant changes in miRNA expression in response to DSS and RJA treatments, identifying potential molecular mechanisms behind RJA's protective effects against DSS-induced colitis.
Project description:The pathogenosis of colitis-associated cancer is unclear. These studies use single nuclear RNA-sequencing on human samples to understand the dominant contributions of cell types to the pathogenesis from histologically normal colon, to colitis, to colitis-associated cancer.
