Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Podocyte-specific transcriptional programs driven by YAP overactivation/YAP5SA overexpression.

ABSTRACT: Podocyte-specific transcriptional programs driven by YAP overactivation/YAP5SA overexpression.

PROVIDER: PRJNA1138722 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR29916202_1.fastq.gz	Fastqsanger.gz
	SRR29916202_2.fastq.gz	Fastqsanger.gz
	SRR29916203_1.fastq.gz	Fastqsanger.gz
	SRR29916203_2.fastq.gz	Fastqsanger.gz
	SRR29916204_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Podocyte-specific transcriptional programs driven by YAP overactivation/YAP5SA overexpression.

Project description:YAP, a transcriptional co-activator, is known to control programs that are both cell- and context-dependent. We report that the overactivation of YAP (YAP5SA) in podocytes has both cell autonomous and non-cell autonomous consequences. RNA-seq analysis of YAP5SA-expressing compared to control podocytes revealed pathways and candidate genes that could explain both these effects.

2025-05-26 | GSE272779 | GEO

RNA-Sequencing of human dermal lymphatic endothelial cells (HDLEC) treated with siRNA targeting YAP/TAZ and YAP5SA

Project description:We analyzed genome-wide transcriptional changes induced by siYAP/TAZ and YAP5SA, using RNA-seq analysis of HDLECs in order to highlight the importance of Hippo/YAP signaling pathway in lymphatic development and maintenance. Here, we report that YAP/TAZ activity is essential for the appropriate lymphatic development and maintenance.

2019-03-03 | GSE116114 | GEO

Single-nucleus-RNA-sequencing data from isolated glomeruli from 4-week-old podocyte-specific YAP or TAZ knockout mice

Project description:YAP and TAZ are paralougous mechanotranducers and act as co-transcription factors pivotal for podocyte homeostasis. We used single nucleus transcriptomic data from podocyte-specific YAP- or TAZ-deficient mice to investigate their unique and shared roles in podocyte health and disease.

2025-06-16 | GSE275614 | GEO

Response of podocytes to PAN - YAP-mediated mechanotransduction determines the podocyte's response to damage

Project description:Podocytes are terminally differentiated cells at the kidney filtration barrier and exposed to considerable mechanical strain. Podocyte injury causes morphological changes as a result of cytoskeletal reorganizations and failure of the filtration barrier. The transcriptional co-activators YAP/TAZ are tightly controlled through hippo signaling and responsive to mechanical cues. Here, we show that YAP is upregulated upon podocyte injury to activate YAP-dependent target genes. This activation preceded the development of proteinuria. In contrast, similar perturbations of cells in culture did not reveal increased YAP activity but showed a downregulation of YAP/TAZ activity when cells were grown on stiff surface. However, culture of cells on soft matrix or inhibition of stress fiber formation allowed recapitulation of the damage-induced YAP upregulation indicating a mechanotransduction-dependent mechanism of YAP over-activity. Interestingly, increased expression of YAP targets was confirmed in renal biopsies from patients with glomerular disease. Consistently, pharmacological inhibition of YAP/TEAD activity ameliorated glomerular disease in vivo. These data suggest that perturbation of the mechanosensitive hippo signaling pathway may be a therapeutic principle in podocyte disease.

2017-04-19 | PXD003872 | Pride

Gene expression analysis of wild type and YAP-activated embryonic mouse stomach

Project description:Hippo-YAP signaling plays important roles in gut development and homeostasis. In this study, we use RNAseq to analyze the gene expression profiles of wild type stomach and YAP5SA-expressing stomach at E13.5. The goal of this study is to understand the transcription program regulated by YAP in the developing gut.

2019-07-30 | GSE103257 | GEO

Increased YAP transcriptional activity in in male murine steroidogenic zG adrenocortical cells

Project description:To specifically evaluate the effect of an increase of the transcriptional activity of YAP in mice adrenal gland we generated a mouse mode expressing a constitutively active form of human YAP (YAP5SA) in aldosterone-producing zG cells. RNAseq analysis on whole adrenal glands were performed on one month male mice

2024-12-30 | GSE233263 | GEO

YAP promotes cardiomyocyte cell cycle entry

Project description:Specialized adult somatic cells, such as cardiomyocytes (CMs), are highly differentiated with poor renewal capacity, an integral reason underlying organ failure in disease and aging. Among the least renewable cells in the human body, CMs renew approximately 1% annually. Consistent with poor CM turnover, heart failure is the leading cause of death. Here, we show that an active version of the Hippo pathway effector YAP, termed YAP5SA, partially reprograms adult mouse CMs to a more fetal and proliferative state. One week after induction, 19% of CMs that enter S-phase do so twice, CM number increases by 40%, and YAP5SA lineage CMs couple to pre-existing CMs. Genomic studies showed that YAP5SA increases chromatin accessibility and expression of fetal genes, partially reprogramming long-lived somatic cells in vivo to a primitive, fetal-like, and proliferative state.

2024-01-11 | GSE152856 | GEO

Effect of YAP5SA expression on the transcriptome of proliferating and senescent cells

Project description:We have generated IMR90-hTERT cells carrying a Doxycyclin-inducible (Tet-On) vector expressing an N-terminally Flag tagged YAP5SA. To identify differentially expressed genes by YAP5SA expression during cellular senescence, we performed transcriptional profiling by RNA sequencing (RNA-seq) with or without YAP5SA expression (using doxycyclin), before and after senescence induction using a stress-induced senescence model (bleomycin-induced senescence).

2025-01-30 | GSE278460 | GEO

Nuclear YAP overexpression drives the human alveolar type 1 cell (AT1) cprogram in a cell autonomous manner in lentiviral transduced iPSC-derived alveolar type 2 cells (iAT2s)

Project description:SPC2B2 iAT2s were transduced with either control WT YAP or constitutively active nuclear YAP (YAP5SA) lentivirus in a single cell suspension and were then grown in iAT2 media (CK + DCI) in 3D matrigel for 7 days.

2023-08-08 | GSE221344 | GEO

Characterization of the interactomes of YAP and TAZ in podocytes identifies a relation between YAP and TAZ and proteins of the nuclear shuttling machinery

Project description:The two effector proteins of the Hippo signaling pathway, YAP and TAZ, play a pivotal role in the cellular homeostasis of podocytes and in the pathogenesis of focal segmental glomerulosclerosis (FSGS). We aim to unravel the unique and redundant functions of YAP and TAZ in the podocyte by identifying podocyte-specific interactors. We generated stable heat sensitive mouse podocytes (hsMPs) carrying a single copy integration of a transgenic construct expressing a flagged version of mouse Yap (3XFLAG.YAP), Taz (3XFLAG.TAZ) or Ruby (3XFLAG.RUBY) in the Rosa26 locus. To explore the interactome of YAP and TAZ in podocytes we immunoprecipitated the tagged proteins and characterized the co-immunoprecipitated protein complexes by mass spectrometry. Within the interactome analyses of the hsMPs, we identified shared and non-shared interacting proteins between YAP and TAZ. Among these identified proteins many well established interactors of YAP and TAZ were included, like proteins of the Tead family, different angiomotins or large tumor suppressor kinase 1 (Lats1). Strikingly, among the shared proteins were numerous proteins of the nuclear shuttling machinery, like importins (Ipo), exportins (Xpo), transportins (Tnpo) and nucleoporins (Nup) that form the nuclear pore complex (NPC), such as NUP107, NUP133, NUP205 and XPO5.

2024-01-26 | PXD040448 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data