Arabidopsis thaliana
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ABSTRACT:
PROVIDER: PRJNA114433 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This SuperSeries is composed of the following subset Series: GSE14646: Gene expression in aerial parts of rice-Arabidopsis Os-LBD37 FOX plants GSE14647: Gene expression in leaf blades of the rice Os-LBD37 overexpressor line RK16331-13 Refer to individual Series
2009-12-26 | E-GEOD-14663 | biostudies-arrayexpress
Project description:Aerial parts of the rice-Arabidopsis FOX (Full-length cDNA overexpressor) lines K16331 and K19624 harboring the rice FL cDNA of LBD37 (Os-LBD37) were analyzed. LBD37 belongs to the plant- specific LOB- (Lateral Organ Boundary) domain family proteins first characterized in Arabidopsis. Results point towards an involvement of the rice LBD37 ortholog of Arabidopsis in nitrogen metabolism- related processes.
2009-12-27 | GSE14646 | GEO
Project description:Aerial parts of the rice-Arabidopsis FOX (Full-length cDNA overexpressor) lines K16331 and K19624 harboring the rice FL cDNA of LBD37 (Os-LBD37) were analyzed. LBD37 belongs to the plant- specific LOB- (Lateral Organ Boundary) domain family proteins first characterized in Arabidopsis. Results point towards an involvement of the rice LBD37 ortholog of Arabidopsis in nitrogen metabolism- related processes. K16331 (T2) and empty vector control plants (T2) were grown for 18 days on solid MS- medium supplemented with 20mg/l Hygromycin. For each hybridization on Affymetrix microarrays, 2 independent plants for K16331 were used. Homozygote K19624 (T3) and empty vector control plants (T3) were grown for 18 days on solid MS- medium. For each hybridization on Affymetrix microarrays, 1 plant was used.
2009-12-26 | E-GEOD-14646 | biostudies-arrayexpress
Project description:We generated more than 23,000 independent Arabidopsis transgenic lines that expressed rice fl-cDNAs (Rice FOX Arabidopsis lines). The short generation time and rapid and efficient transformation frequency of Arabidopsis enabled the functions of the rice genes to be analyzed rapidly. We screened rice FOX Arabidopsis lines for alterations in morphology, photosynthesis, element accumulation, pigment accumulation, hormone profiles, secondary metabolites, pathogen resistance, salt-tolerance, UV signaling, high light tolerance, and heat stress tolerance. Since we isolated 6 genes (AK069096, AK073112, AK073675, AK065297, AK102323 and AK073210) causing an increase in the abundance of metabolites or pigments detected by absorption data, we investigated the expression profiles in transgenic plants by using microarray analysis. Expression of the genes encoding chalcone synthase and dihydroflavonol-4-reductase was increased in all 6 cDNA transformants. These results indicate that an increase in expression of the genes encoding flavonoid biosynthesis enzymes caused an accumulation of pigments in these 6 transformants. Experiment Overall Design: Total RNA was isolated from aerial parts of re-transformants of some rice cDNAs and wild type using an RNAqueous Kit (Ambion, Inc, USA).
2008-12-05 | E-GEOD-13165 | biostudies-arrayexpress
Project description:To understand how GTL1 regulates cell growth, we first identified its potential direct targets by the chromatin immunoprecipitation followed by the hybridization on an Affymetrix Arabidopsis Tiling 1.0R array (ChIP-chip). To enrich the genomic region bound by GTL1 in vivo, we harvested whole aerial parts of 12-day-old gtl1-1 plants complemented with the pGTL:GTL1:GFP constructs and immunoprecipitated the chromatin fragments associated with GTL1-GFP proteins using antibodies against GFP. After applying a cut-off P-values of 0.001of MAT (Model-based analysis of tiling array), we identified a total number of 3,900 putative immediate target genes that showed consistent binding by GTL1.
2013-08-01 | GSE40519 | GEO
Project description:When aboveground parts of intact plants are exposed to volatile organic compounds emitted from neighboring con-/heterospecific plants that are artificially damaged or damaged by herbivores, the resistant responses are induced in the plants. Differential responses of plants to enantiomers of the same volatile compound have also been reported in Arabidopsis: the root became shorter when Arabidopsis seedlings are exposed to aerial borneol, and the dose-dependent root length reduction was significantly different between (+)- and (-)-borneol. We identified (+)-borneol dependent inductive genes in Arabidpsis in this transcriptome analysis.
2023-04-01 | GSE180220 | GEO
Project description:Cytokinin treatment on aerial parts of seedlings
| PRJNA99159 | ENA
Project description:Iron (Fe) is an essential nutrient for virtually all plants that modulate immune responses. However, whereas low and high Fe availalbility confers susceptibility to the fungus Colletotrichum higgisianum, the same conditions lead to rice tolerance to Magnaporthe oryzae. To better understand the systemic responses behind this antagonistic pattern, transcriptome profiling on fungal infected Arabidopsis and rice plants has been conducted. To consider the signaling component and avoid the interference of tissues experiencing necrosis, samples were conducted on aerial tissues 48h post-infection, when both pathogens remain in the biotrophic phase. Collectively, our data support that Arabidopsis employs a defence strategy based on glucosinolates and other secondary metabolites, whereas rice uses oxidative stress based mechanisms. A different usage of Fe pools during infection is also proposed.
2025-01-26 | GSE231365 | GEO
Project description:Expression data from aerial parts of 14-day-old wild-type and GFP-TIE1-15 Arabidopsis seedlings
| PRJNA167546 | ENA
Project description:Arabidopsis sfr mutants are deficient in cold acclimation during exposure to coolnon-freezing temperatures. Although not visibly affected by the cold they have lost the ability to survive subsequent freezing. We plan to investigate how the sfr2 and sfr6 mutants respond to low temperature on the gene expression level. Wild type plants that have undergone identical treatments in parallel are necessary controls. The cold treatment of plants in the rosette stage (soil grown in a 8/16 hours day/night cycle) will be carried out in a cooled growth chamber at 4 degrees for 24 hours (same light regimetreatment starting/ending at the 4th hour of light). The aerial parts of the treated and untreated plants will be collected and frozen immediately in liquid nitrogen for RNA extraction. Comparison of the cold response of thousands of Arabidopsis genes in the wild type to the situation in our freezing sensitive mutants will enhance our understanding of the cold response itself and illuminate the effect of the mutations on the cold acclimation process. Experimenter name = Irene Bramke Experimenter phone = 01784 44 3770 Experimenter fax = 01784 43 4326 Experimenter address = Royal Holloway Experimenter address = University of London Experimenter address = School of Biological Sciences Experimenter address = Bourne Building Experimenter address = Laboratory 406 Experimenter zip/postal_code = TW20 OEX Experimenter country = UK Keywords: growth_condition_design; genetic_modification_design
2007-01-08 | GSE5711 | GEO