Adhesion-Related Macrophages Regulates Metabolic Homeostasis through CAV-1 Dependency
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PROVIDER: PRJNA1145453 | ENA |
REPOSITORIES: ENA
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Project description:Adhesion-Related Macrophages Regulate Metabolic Homeostasis through CAV-1 Dependency
| PRJNA1414483 | ENA
Project description:The significance of adipose tissue macrophages (ATMs) in regulating adipose tissue function is well-established. However, our investigation revealed a previously overlooked subpopulation of macrophages adhered to adipocytes, which we term adhesion-related macrophages (ARMs). We developed an approach to isolate ARMs and compared them with SVF macrophages (SMs). Our findings demonstrate that ARMs constitute the predominant expanded subpopulation of ATMs during obesity, ARMs acquire adipocyte mRNA through direct adhesion to adipocytes, thereby enhancing their lipid processing capacity. Notably, ARMs can be characterized by a key functional marker, Caveolin-1. Genetic ablation of Caveolin-1 in macrophages significantly diminishes ARM abundance, disrupting their adhesion capacity and lipid content, leading to adipocyte hypertrophy, adipose tissue expansion, and impaired glucose homeostasis. Reintroducing ARMs from lean mice into eWAT mitigate obesity-induced insulin resistance. Our study uncovers ARM as a potential therapeutic target for obesity-induced insulin resistance and opening avenues for identifying similar paradigms in other tissues and diseases.
2026-03-31 | GSE317566 | GEO
Project description:The significance of adipose tissue macrophages (ATMs) in regulating adipose tissue function is well-established. However, our investigation revealed a previously overlooked subpopulation of macrophages adhered to adipocytes, which we term adhesion-related macrophages (ARMs). We developed an approach to isolate ARMs and compared them with traditional macrophages from the stromal vascular fraction. Our findings demonstrate that ARMs constitute the predominant expanded subpopulation of adipose tissue macrophages during obesity, exhibiting heightened adhesion, proliferation, and lipid-processing capacities. Notably, ARMs can be characterized by a key functional marker, Caveolin-1. Genetic ablation of Caveolin-1 in immune cells significantly diminishes ARM abundance, disrupting their adhesion capacity and lipid content, leading to adipocyte hypertrophy, adipose tissue expansion, and impaired glucose homeostasis. Reintroducing ARMs from lean mice into eWAT mitigate obesity-induced adipose tissue inflammation and insulin resistance. Our studies uncover a previously unexplored macrophage subpopulation, ARMs, revealing potential therapeutic targets for obesity-induced insulin resistance and opening avenues for identifying similar paradigms in other tissues and diseases.
2025-08-07 | GSE274245 | GEO
Project description:Brain gene transfer using viral vectors will likely become a therapeutic option for several disorders. Helper-dependent (HD) canine adenovirus type 2 vectors (CAV-2) are well suited for this goal. Indeed, these vectors are poorly immunogenic, efficiently transduce neurons, are retrogradely transported to afferent structures in the brain, and lead to long-term transgene expression. CAV-2 vectors have been exploited to unravel behavior, cognition, neural networks, axonal transport and therapy for orphan diseases. Here we describe the HD-CAV-2 vector induced transcriptional response of human dopaminergic neurospheres derived from midbrain progenitors. In this 3D model system, brain cell functions and dynamics mimic several aspects the dynamic nature of human brain. With the goal of better understanding and characterizing HD CAV-2 for brain therapy, we analyzed the transcriptomic modulation induced by HD-CAV-2 in this brain model system. We found that dopaminergic neurospheres are readily transduced by HD-CAV-2, and that transduction generates two main responses: a DNA damage response, and alteration of centromeric and microtubule probes. We suggest that the first effect is linked to viral DNA, while the second would be related to the interaction of the HD-CAV-2 fibre with CAR.
2014-10-24 | GSE62687 | GEO
Project description:Brain gene transfer using viral vectors will likely become a therapeutic option for several disorders. Helper-dependent (HD) canine adenovirus type 2 vectors (CAV-2) are well suited for this goal. Indeed, these vectors are poorly immunogenic, efficiently transduce neurons, are retrogradely transported to afferent structures in the brain, and lead to long-term transgene expression. CAV-2 vectors have been exploited to unravel behavior, cognition, neural networks, axonal transport and therapy for orphan diseases. Here we describe the HD-CAV-2 vector induced transcriptional response of human dopaminergic neurospheres derived from midbrain progenitors. In this 3D model system, brain cell functions and dynamics mimic several aspects the dynamic nature of human brain. With the goal of better understanding and characterizing HD CAV-2 for brain therapy, we analyzed the transcriptomic modulation induced by HD-CAV-2 in this brain model system. We found that dopaminergic neurospheres are readily transduced by HD-CAV-2, and that transduction generates two main responses: a DNA damage response, and alteration of centromeric and microtubule probes. We suggest that the first effect is linked to viral DNA, while the second would be related to the interaction of the HD-CAV-2 fibre with CAR. Total RNAs extracted from 3D cultures of transduced hmNPCs cells at 2 h and 5 days post-infection with HD-CAV-2 vector were hybridized on Affymetrix microarrays and pair wise comparisons were performed between the mock and vector transduced hmNPCs cells. Each condition was tested in three replicates.
2014-10-24 | E-GEOD-62687 | biostudies-arrayexpress
Project description:Chicken anaemia virus (CAV) causes severe anaemia and immunosuppression in young chickens. Such effects reduce the efficiency of routine vaccinations while aggravating the effects of other pathogens in chicken populations. So far, the host responses to CAV have not been studied in vivo or in vitro on a whole genome-wide scale. In this study, we compared gene expression profiles of uninfected and CAV infected cell cultures (MDCC-MSB1 cells-in vitro) at 24, 48 and 72 hr post infection. A chicken specific-immune microarray (5k) developed at Roslin Institute was used throughout the study. Statistically significant gene expression changes occurred mainly after 72 hr (in vitro).
2009-08-20 | E-MEXP-1229 | biostudies-arrayexpress
Project description:Artemisia arbuscula strain:CAV-1 Transcriptome or Gene expression
| PRJNA258193 | ENA
Project description:Chicken anaemia virus (CAV) causes severe anaemia and immunosuppression in young chickens. Such effects reduce the efficiency of routine vaccinations while aggravating the effects of other pathogens in chicken populations. So far, the host responses to CAV have not been studied in vivo on a whole genome-wide scale. In this study, we compared gene expression profiles of chicken tissues (thymus, bone marrow, bursa of Fabricius and spleen-in vivo) from SPF chickens at 14-day-old following intramuscular inoculation at day-old. A chicken specific-immune microarray (5k) developed at Roslin Institute was used throughout the study. Statistically significant gene expression changes occurred mainly in the thymus (in vivo).
2008-08-21 | E-MEXP-1228 | biostudies-arrayexpress
Project description:We observed functional outcomes of cav-1 knockdown and wanted to characterize changes in gene expression profiles following this manipulation. To address this, we treated nematodes with daf-2 or cav-1 RNAi and compared gene expression profiles to these of untreated worms. The knockdown of cav-1 resulted in modulation in the expression of 92 genes, and some of them overlapped with genes which were affected by knockdown of daf-2.
2017-06-19 | GSE100045 | GEO
Project description:Transcriptional Rewiring of BET Inhibitor Treated Ewing Sarcoma Cells Augments their Dependency on Focal Adhesion Kinase
| PRJNA1274418 | ENA